Vascularized thymic lobe transplantation in miniature swine: I. Vascularized thymic lobe allografts support thymopoiesis

Transplantation
John C LaMattinaKazuhiko Yamada

Abstract

Vascularized thymokidney transplants have previously been shown to induce tolerance across major histocompatibility complex barriers. The ability to perform vascularized thymic lobe transplantation could permit such tolerance to be induced with any cotransplanted solid organ or tissue. For this reason, we have developed a technique for vascularized thymic lobe transplantation in miniature swine. Thymic vessels (n=2) were anastomosed to the carotid artery and the external jugular vein of naïve minor-mismatched recipients treated with a 12-day course of cyclosporine A (10 mg/kg/day). Graft survival and thymopoiesis were assessed by histology, immunohistochemistry, and fluorescence-activated cell sorting. Allele-specific antibodies 74-12-4 and pig allelic antigen (PAA) were used to distinguish donor and recipient cells. Allografts showed intact cortical and medullary structure posttransplantation, without evidence of rejection or ischemia. Recipient thymocytes repopulated the donor cortical thymus by POD30 and increased in the cortex and medulla by POD60. Our study demonstrates the technical feasibility of vascularized thymic lobe transplantation and the support of thymopoiesis by such transplants in a large animal model. This tec...Continue Reading

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Citations

Jan 21, 2012·Current Opinion in Organ Transplantation·Kazuhiko Yamada, Joseph Scalea
Jun 18, 2010·Transplantation·Adam D GriesemerMark A Hardy
May 24, 2012·Biotechnology & Genetic Engineering Reviews·M'Balu A WebbRoger F James
Dec 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shuji NoboriKazuhiko Yamada
Mar 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chisako KamanoKazuhiko Yamada
Jun 6, 2012·Transplantation Proceedings·D ZhaoL Hong
Dec 9, 2008·Transplant Immunology·David H SachsKazuhiko Yamada
Aug 25, 2015·International Journal of Surgery·Parsia A VagefiDavid H Sachs
Sep 12, 2006·The Surgical Clinics of North America·Matthew J WeissJoren C Madsen
May 25, 2005·Pediatric Transplantation·Dax A Guenther, Joren C Madsen
Dec 18, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Matthew T MenardJoren C Madsen
Aug 16, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Nicolas J MuellerJay A Fishman
Mar 1, 2012·Xenotransplantation·Joseph ScaleaKazuhiko Yamada
Feb 13, 2014·Immunological Reviews·Adam GriesemerMegan Sykes
Aug 10, 2010·Transplant Immunology·Atsushi HirakataKazuhiko Yamada
May 5, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M TasakiK Yamada
Sep 25, 2017·Current Opinion in Organ Transplantation·Kazuhiko YamadaDavid H Sachs
May 2, 2020·Cell and Tissue Research·Reinhard Pabst

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