Apr 21, 2016

Vasculogenesis Potential of Mesenchymal and Endothelial Stem Cells Isolated from Various Human Tissues.

BioRxiv : the Preprint Server for Biology
Rokhsareh RohbanThomas Pieber

Abstract

Neo vessel formation can be initiated by co-transplantation of mesenchymal stem cells (MSC) with endothelial colony-forming cells (ECFC). The two adult stem cell types can be isolated and expanded from a variety of tissues to be used for regenerative applications pro-angiogenesis. Here we performed a systematic study to evaluate the neo-vasculogenesis potential of MSC and ECFC isolated from various human tissues. MSC were isolated, purified and expanded in vitro from umbilical cord (UC) and umbilical cord blood (UCB), white adipose tissue (WAT), bone marrow (BM), and amniotic membrane of placenta (AMN). ECFC were isolated from UC and UCB, WAT and peripheral blood (PB). ECFC and MSC and were co-transplanted admixed with extracellular matrix (Matrigel) at a ratio of 5:1 to immune-deficient NSG mice, subcutaneously. The transplants were harvested after two weeks and the state of vessel formation and stability in the explants were investigated using immune-histochemical methods. The number of created micro-vessels was quantified using Hematoxylin & Eosin (H&E) staining followed by image J quantification. Results showed that ECFC and MSC possess variable capacity in contributing to neo-vasculogenesis. WAT and UCB-derived ECFC and WA...Continue Reading

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Mentioned in this Paper

Study
In Vivo
Co-treatment
Strategy
Matrigel
Angiogenic Process
Peripheral Blood
Placenta Specimen
Vasculogenesis
Blood Vessel

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