Vasectomy effect on canine seminal plasma biochemical components and their correlation with seminal parameters

Theriogenology
Fabiana Ferreira de SouzaMaria Denise Lopes

Abstract

Three semen samples were collected at 48 h intervals from 20 mature research dogs previously conditioned to manual semen collection. Vasectomy was performed in all dogs, and 15 days after surgery, another three ejaculates were similarly collected. The semen was evaluated, and centrifuged to obtain seminal plasma for measurement of pH, and concentrations of total proteins (TP), total chlorides (Cl), calcium (Ca), potassium (K), and sodium (Na). The seminal plasma protein profile was evaluated by SDS-PAGE; molecular weights and the integrated optical density (IOD) of each band were estimated. There was a negative correlation between K concentration and progressive motility (r = -0.49, P = 0.027), sperm vigor (r = -0.60, P = 0.0053), and plasma integrity, evaluated by both the hypo-osmotic swelling test (r = -0.50, P = 0.026) and a fluorescent stain (r = -0.45, P = 0.046). Positive correlations between Na and K pre- and post-vasectomy (r = 0.88, P < 0.001; r = 0.56, P < 0.01, respectively) were verified. There were a total of 37 bands pre-vasectomy and 35 post-vasectomy (range, 100.6-3.6 kDa). Bands B9 and B13 (42.6 and 29.2 kDa) were not present post-vasectomy. The IOD of band B3 (73.5 kDa) was higher (P = 0.03) pre-vasectomy, co...Continue Reading

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Citations

May 8, 2007·Theriogenology·M V Root Kustritz
Nov 28, 2019·Veterinary Clinical Pathology·Anne-Kathrin UmbachAxel Wehrend
Aug 15, 2019·The Veterinary Record·Arantza VitoriaFrancisco José Vazquez
Dec 11, 2019·Animals : an Open Access Journal From MDPI·Silvan R Urfer, Matt Kaeberlein
May 17, 2019·Reproduction in Domestic Animals = Zuchthygiene·Anne-Kathrin UmbachAxel Wehrend
Nov 4, 2020·International Journal of Molecular Sciences·Melissa Carvajal-SernaAdriana Casao
Mar 4, 2020·Journal of Proteomics·Ingrid MillerElisabetta Gianazza

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