Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease

Atherosclerosis
Meegan WalkerChristopher D Askew

Abstract

Peripheral arterial disease (PAD) is characterised by impaired leg blood flow, which contributes to claudication and reduced exercise capacity. This study investigated to what extent vasoactive enzymes might contribute to altered blood flow in PAD (Fontaine stage II). We compared femoral artery blood flow during reactive hyperaemia, leg-extension exercise and passive leg movement, and determined the level of vasoactive enzymes in skeletal muscle samples from the vastus lateralis in PAD (n = 10, 68.5 ± 6.5 years) and healthy controls (CON, n = 9, 62.1 ± 12.3 years). Leg blood flow was measured with Doppler ultrasound and muscle protein levels of phosphorylated endothelial nitric oxide synthase, NADPH oxidase, cyclooxygenase 1 and 2, thromboxane synthase, and prostacyclin synthase were determined. Leg blood flow during the initial 90 s of passive leg movement (242 ± 33 vs 441 ± 75 ml min(-1), P = 0.03) and during reactive hyperaemia (423 ± 100 vs 1255 ± 175 ml min(-1), P = 0.002) was lower in PAD than CON, whereas no significant difference was observed for leg blood flow during exercise (1490 ± 250 vs 1887 ± 349 ml min(-1), P = 0.37). PAD had higher NADPH oxidase than CON (1.04 ± 0.19 vs 0.50 ± 0.06 AU, P = 0.02), with no differe...Continue Reading

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Apr 8, 2017·Journal of Applied Physiology·J Carter LuckMatthew D Muller
Jun 23, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Corey R HartRussell S Richardson
Jul 3, 2019·Sports Medicine·Joel D Trinity, Russell S Richardson
Aug 11, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Annelise L MenesesChristopher D Askew
Dec 31, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Jacqueline K LimbergJaume Padilla
Nov 13, 2019·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Gianni PariseMichael J Plyley
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Mar 25, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Oliver BaumYlva Hellsten
Aug 31, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Alec L E ButenasSteven W Copp
Dec 12, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Joel D TrinityRussell S Richardson

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