Abstract
Adenosine is an important inhibitory neuromodulator in the cerebral cortex, yet it remains unclear how extracellular adenosine concentrations are regulated. Recently, it has been shown that beta-adrenergic receptor stimulation in rat cortical cultures causes the accumulation of extracellular adenosine derived by enzymatic hydrolysis from adenosine cyclic monophosphate (cAMP) transported into the extracellular space. In this study we show that vasoactive intestinal peptide (VIP), in addition to activating adenylyl cyclase and promoting the accumulation of intracellular cAMP in rat cortical cultures, also causes transport of cAMP and accumulation of extracellular adenosine. We further show that the extracellular accumulation of adenosine in response to VIP can be blocked by inhibition of cAMP transport, cyclic nucleotide phosphodiesterase activity, and 5'-nucleotidase, indicating that extracellular cAMP is the source of the adenosine. Cyclic AMP transport may be a general mechanism by which a variety of neuromodulators that act upon receptors coupled to adenylyl cyclase might regulate extracellular adenosine levels and thereby inhibitory tone in the cerebral cortex.
References
Sep 1, 1992·Brain Research Bulletin·P A Rosenberg
Jan 1, 1991·Trends in Pharmacological Sciences·C D NicholsonM Shahid
Jan 1, 1990·British Journal of Pharmacology·R A Challiss, C D Nicholson
Jul 1, 1987·Journal of Neurochemistry·A M Etgen, E T Browning
Mar 1, 1985·Journal of Neurochemistry·H ChneiweissJ Prémont
Sep 1, 1985·Journal of Neurochemistry·W F MacDonald, T D White
Jan 16, 1987·Journal of Chromatography·D Perrett
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P J MagistrettiF E Bloom
Oct 20, 1983·Nature·G RougonA W Mudge
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K Tatemoto, V Mutt
Apr 1, 1994·Trends in Pharmacological Sciences·T Harmar, E Lutz
May 1, 1994·Neuron·R W Gereau, P J Conn
Citations
Jun 14, 1996·Neuroscience Letters·P A Rosenberg, Y Li
Feb 5, 1999·Biochemical Pharmacology·C FlorioR M Gaion
Sep 6, 2000·Trends in Pharmacological Sciences·A M Sebastião, J A Ribeiro
Aug 15, 2001·Neuroscience Letters·P Correia-de-SáJ A Ribeiro
Feb 28, 2003·Neuroscience·H J BennettK Semba
Dec 7, 2007·Neuroscience Letters·Diana Cunha-ReisAna M Sebastião
Jul 28, 2007·Peptides·Olfa Masmoudi-KoukiMarie-Christine Tonon
May 19, 2004·The European Journal of Neuroscience·Yin LuPaul A Rosenberg
Apr 3, 2003·The European Journal of Neuroscience·Yin LuPaul A Rosenberg
Oct 27, 2004·Neuroscience Letters·Minou LePaul A Rosenberg
Oct 13, 2006·Neuropharmacology·D Cunha-ReisA M Sebastião
Sep 13, 2001·American Journal of Physiology. Renal Physiology·E K Jackson, R K Dubey
Nov 10, 2001·Journal of Neurochemistry·S Latini, F Pedata
Jul 21, 2001·Annals of the New York Academy of Sciences·F PedataS Latini