Vasoactive intestinal peptide (VIP)-mediated expression and function of steroidogenic acute regulatory protein (StAR) in granulosa cells.

Molecular and Cellular Endocrinology
Mariusz P KowalewskiDouglas M Stocco

Abstract

VIP is a peptide hormone capable of activating the cAMP/PKA pathway and modifying gonadal steroidogenic capacity. Less is known about the molecular mechanisms of VIP-mediated steroidogenesis and its role in regulating the steroidogenic acute regulatory protein (STAR). We examined the impact of VIP on STAR expression and function in immortalized (KK1) and primary mouse granulosa cells, where VIP strongly upregulated STAR expression and steroidogenesis. Inhibitors of the PKA and PKC pathways suggested that both are activated by VIP. VIP did not efficiently phosphorylate STAR (P-STAR); however, VIP together with cAMP-analogs that activate Type II PKA increased P-STAR and further increased steroidogenesis. Our results suggest that VIP-induced STAR expression and function in granulosa cells result from the preferential activation of Type I PKA. Furthermore, the PKA and PKC pathways appear to converge at regulating VIP-mediated Star transcription and translation.

References

Jul 15, 1979·FEBS Letters·P RobberechtJ Christophe
Sep 30, 1977·Science·L I LarssonO B Schaffalitzky de Muckadell
Aug 29, 1998·Molecular and Cellular Endocrinology·F El-GehaniI Huhtaniemi
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·H S BoseW L Miller
Feb 17, 2006·Theriogenology·Mariusz Pawel KowalewskiBernd Hoffmann
Jan 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Claudio ParraHernán E Lara
Jul 31, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Marzia BarberiRita Canipari

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Citations

Jan 15, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Mariusz P KowalewskiIris Reichler
Oct 22, 2015·Molecular Human Reproduction·S StreiterR Abir
Aug 5, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Aykut GramMariusz P Kowalewski
Dec 6, 2014·Molecular and Cellular Endocrinology·Mariusz Pawel KowalewskiAlois Boos
Oct 22, 2014·Endocrine·Gabriela RosasLeticia Morales-Ledesma
Jun 21, 2014·Reproduction in Domestic Animals = Zuchthygiene·A GramM P Kowalewski
Mar 7, 2020·JPEN. Journal of Parenteral and Enteral Nutrition·Ting ChenJingci Zhu

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