PMID: 3746700May 1, 1986Paper

Vasoactive intestinal polypeptide depolarizations in cat bladder parasympathetic ganglia

The Journal of Physiology
T AkasuP Shinnick-Gallagher

Abstract

The effect of vasoactive intestinal polypeptide (VIP) on the neuronal membranes of isolated cat vesical pelvic ganglia and its underlying ionic mechanism were examined by means of intracellular recording and voltage-clamp techniques. Application of VIP (0.05-50 microM) to the neurones by pressure 'puff' ejection through a micropipette placed close to the neurones produced a depolarizing response (2-15 mV) in 83% of neurones tested; this effect was concentration dependent. The VIP-induced depolarization frequently evoked spontaneous action potentials in quiescent neurones and increased the frequency of action potentials in spontaneously firing neurones. The VIP depolarization was not blocked in a Ca2+-free, high-Mg2+ solution or in a solution containing hexamethonium (1 mM) and atropine (1 microM). Tetrodotoxin (TTX; 1 microM) also did not affect the VIP depolarization. The VIP depolarization was associated with an increase in membrane resistance and the slope of a current-voltage relation (I-V curve) was increased by VIP. Conditioning hyperpolarization and depolarization of the membrane increased and decreased the amplitude of the VIP depolarization, respectively. The VIP depolarization reversed polarity around--100 mV. The rev...Continue Reading

Citations

May 29, 2000·The Bulletin of Tokyo Dental College·K HayashiT Suzuki
Apr 1, 1995·Progress in Neurobiology·T Akasu, T Nishimura
May 1, 1990·Pflügers Archiv : European journal of physiology·E Kumamoto, P Shinnick-Gallagher
Jun 1, 1989·Pflügers Archiv : European journal of physiology·E Kumamoto
Mar 1, 1997·International Journal of Urology : Official Journal of the Japanese Urological Association·N Yoshimura, W C de Groat
Jun 11, 2021·Scientific Reports·Krystyna MakowskaSlawomir Gonkowski

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