Vasodilator-stimulated phosphoprotein (VASP) regulates actin polymerization and contraction in airway smooth muscle by a vinculin-dependent mechanism.

The Journal of Biological Chemistry
Yidi Wu, S J Gunst

Abstract

Vasodilator-stimulated phosphoprotein (VASP) can catalyze actin polymerization by elongating actin filaments. The elongation mechanism involves VASP oligomerization and its binding to profilin, a G-actin chaperone. Actin polymerization is required for tension generation during the contraction of airway smooth muscle (ASM); however, the role of VASP in regulating actin dynamics in ASM is not known. We stimulated ASM cells and tissues with the contractile agonist acetylcholine (ACh) or the adenylyl cyclase activator, forskolin (FSK), a dilatory agent. ACh and FSK stimulated VASP Ser(157) phosphorylation by different kinases. Inhibition of VASP Ser(157) phosphorylation by expression of the mutant VASP S157A in ASM tissues suppressed VASP phosphorylation and membrane localization in response to ACh, and also inhibited contraction and actin polymerization. ACh but not FSK triggered the formation of VASP-VASP complexes as well as VASP-vinculin and VASP-profilin complexes at membrane sites. VASP-VASP complex formation and the interaction of VASP with vinculin and profilin were inhibited by expression of the inactive vinculin mutant, vinculin Y1065F, but VASP phosphorylation and membrane localization were unaffected. We conclude that V...Continue Reading

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Oct 5, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katelynn M Wilton, Daniel D Billadeau
Apr 11, 2019·Cell Biology International·Patrycja Rachubik, Agnieszka Piwkowska
Jan 16, 2020·American Journal of Respiratory Cell and Molecular Biology·Chun Y Seow, Steven S An
Sep 25, 2016·The Journal of Biological Chemistry·Jia LiDale D Tang
Mar 20, 2021·European Journal of Pharmacology·Peter J MoroneJoyce Cheung-Flynn
Aug 10, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yinna WangDale D Tang

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