PMID: 2107881Apr 2, 1990Paper

Vasopressin and norepinephrine stimulation of inositol phosphate accumulation in rat hepatocytes are modified differently by protein f1nase C and protein kinase A

Biochimica Et Biophysica Acta
R A Pittner, J N Fain

Abstract

Rat hepatocytes were maintained in primary monolayer culture for 24 h in the presence of serum. Treatment of hepatocytes with 1 microM 4 beta-phorbol 12 beta-myristate 13 alpha-acetate (PMA) for 5-15 min increased membrane-associated protein kinase C activity and concomitantly decreased soluble activity. Membrane protein kinase C activity returned to basal values within 1 h then decreased by more than 50% within 2 h. Prolonged (2-18 h) incubation with PMA did not further decrease protein kinase C activity. Pretreatment of hepatocytes with PMA for 5-15 min had little effect on the subsequent actions of 100 nM vasopressin but abolished the stimulation of inositol phosphate accumulation by 3 nM vasopressin and 20 microM norepinephrine. Long-term exposure (2-18 h) of hepatocytes to 1 microM PMA actually enhanced the effects of vasopressin and 20 microM norepinephrine. The stimulation by norepinephrine (20 microM) of inositol phosphate accumulation was abolished by the alpha 1-adrenergic antagonist prazosin (1 microM), whereas the beta-adrenergic antagonist propranolol (30 microM) had little effect. Addition of 8Br-cAMP (100 microM) or glucagon (10 nM) for 5 min or 8 h had no significant effect alone, but enhanced the subsequent vas...Continue Reading

References

Apr 2, 1990·Biochimica Et Biophysica Acta·R A Pittner, J N Fain
Jul 18, 1986·Science·Y Nishizuka
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A D WhettonC P Downes
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Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N G MorganJ H Exton

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Citations

Apr 1, 1993·The American Journal of Physiology·R A Pittner, J A Spitzer
Apr 2, 1990·Biochimica Et Biophysica Acta·R A Pittner, J N Fain
Jun 12, 1990·Biochimica Et Biophysica Acta·J N Fain

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