Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation

Scientific Reports
G DayanithiAlexei Verkhratsky

Abstract

The neurohormones arginine-vasopressin (AVP) and oxytocin (OT) synthesised in supraoptic and paraventricular nuclei of neurohypophysis regulate lactation, systemic water homeostasis and nociception. Using transgenic rats expressing AVP and OT tagged with fluorescent proteins we demonstrate that both neurohormones are expressed in sensory neurones both in vitro, in primary cultures, and in situ, in the intact ganglia; this expression was further confirmed with immunocytochemistry. Both neurohormones were expressed in nociceptive neurones immunopositive to transient receptor potential vannilloid 1 (TRPV1) channel antibodies. The AVP and OT-expressing DRG neurones responded to AVP, OT, 50 mM K+ and capsaicin with [Ca2+]i transients; responses to AVP and OT were specifically blocked by the antagonists of V1 AVP and OT receptors. Probing the extracellular incubation saline with ELISA revealed AVP and OT secretion from isolated DRGs; this secretion was inhibited by tetanus toxin (TeNT) indicating the role for vesicular release. Expression of OT, but not AVP in DRG neurones significantly increased during lactation. Together, the results indicate novel physiological roles (possibly related to nociception and mood regulation) of AVP and...Continue Reading

References

Jun 1, 1989·The Journal of Comparative Neurology·M G GarryV S Seybold
Jan 1, 1987·Experimental Brain Research·A M Horn, S L Lightman
Jun 23, 1987·Brain Research·M VecsernyésF A László
Sep 8, 2000·Archives of Pharmacal Research·Y S Kang, J H Park
Jan 20, 2009·Journal of Pharmacological Sciences·Taka-aki Koshimizu, Gozoh Tsujimoto
Jun 13, 2009·Molecular and Cellular Neurosciences·Christina F VogelaarJames W Fawcett
Jun 12, 2010·Stress : the International Journal on the Biology of Stress·Miwako TodorokiShoji Nagata
Jun 18, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ara Schorscher-PetcuJeffrey S Mogil
Jul 16, 2010·CNS Neuroscience & Therapeutics·Cedric VieroGovindan Dayanithi
Oct 25, 2011·Nature Neuroscience·Jeffrey S MogilRoger B Fillingim
Mar 6, 2012·Cell Calcium·Govindan DayanithiEmil C Toescu
Aug 6, 2013·Journal of Physiology and Biochemistry·Ahmet AyarHaluk Kelestimur
Oct 18, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Pierre-Eric JuifPierrick Poisbeau
Oct 30, 2014·Neuromodulation : Journal of the International Neuromodulation Society·Elliot S Krames
Nov 18, 2016·Pharmaceuticals·Aaron D MickleDurga P Mohapatra
Mar 10, 2018·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Rafael Taeho HanHeung Sik Na

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Citations

Aug 27, 2021·Frontiers in Neuroendocrinology·Haiyan ZhengSun Wook Hwang
Oct 30, 2021·Journal of Neuroendocrinology·Yoichi Ueta

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Methods Mentioned

BETA
transgenic
ELISA
biopsies
FCS
dissection
Enzyme-Linked Immunosorbent Assay

Software Mentioned

Origin
MATLAB
MathWorks Statistics Toolbox
ImageJ
Axio Vision4

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