Vasopressin potentiates corticotropin-releasing hormone-induced insulin release from mouse pancreatic beta-cells.

The Journal of Endocrinology
Anne-Marie O'CarrollStephen J Lolait

Abstract

Arginine vasopressin (AVP) and corticotropin-releasing hormone (CRH) have both been implicated in modulating insulin secretion from pancreatic beta-cells. In the present study, we investigated the insulin-secreting activities of AVP and CRH in wild-type and AVP VIb receptor knockout mice. Both neuropeptides stimulated insulin secretion from isolated mouse pancreatic islets. The response of islets to CRH was increased fourfold by concomitant incubation with a subthreshold dose of AVP that alone did not stimulate insulin secretion. Activation of the endogenously expressed M3 receptor by the cholinergic agonist carbachol also potentiated CRH-induced insulin secretion, indicating that the phenomenon may be pathway specific (i.e. Ca2+-phospholipase C) rather than agonist specific. The protein kinase C (PKC) inhibitors Ro-31-8425 and bisindolylmaleimide I attenuated the potentiating effect of AVP on CRH-stimulated insulin secretion and blocked AVP-stimulated insulin secretion. A possible interaction between the PKC and protein kinase A pathways was also investigated. The phorbol ester phorbol myristate acetate (PMA) stimulated insulin secretion, while the addition of both PMA and CRH enhanced insulin secretion over that measured with...Continue Reading

References

Nov 1, 1988·The American Journal of the Medical Sciences·J A AmicoJ Haldar
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·S J LolaitM J Brownstein
Jul 26, 1995·Biochemical and Biophysical Research Communications·M SaitoH Kawashima
Oct 26, 2002·Molecular Psychiatry·S R WersingerW S Young
May 9, 2003·American Journal of Physiology. Endocrinology and Metabolism·Viviane FolnyClaudine Serradeil-Le Gal
Oct 24, 2003·Cell Calcium·Jason I E BruceDavid I Yule
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Tracy L Bale, Wylie W Vale
Feb 27, 2004·Biochemical and Biophysical Research Communications·Walter S ZawalichMinoru Matsui
Jun 24, 2004·American Journal of Reproductive Immunology : AJRI·Sophia N KalantaridouGeorge P Chrousos
Sep 1, 2005·The Journal of Biological Chemistry·Blanca RubíPierre Maechler
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Chien LiWylie Vale

❮ Previous
Next ❯

Citations

Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mark O HuisingWylie W Vale
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Janine SchmidStefan R Bornstein
Jun 19, 2009·Current Opinion in Gastroenterology·Rashmi Chandra, Rodger A Liddle
Mar 19, 2014·Molecular and Cellular Endocrinology·Gerald ThielOliver G Rössler
May 23, 2013·Pharmacology & Therapeutics·Stefan AmistenShanta J Persaud
Sep 11, 2010·Stress : the International Journal on the Biology of Stress·Ja RoperSj Lolait
Oct 18, 2012·Physiological Reviews·Taka-aki KoshimizuAkito Tanoue
Jun 27, 2019·Current Obesity Reports·Cherlyn Ding, Faidon Magkos
Dec 22, 2016·American Journal of Physiology. Endocrinology and Metabolism·Christopher TaveauNadine Bouby
Jul 22, 2018·Endocrine·Giovanna MuscogiuriSilvia Savastano
Apr 28, 2021·Peptides·Mitsuhiro YoshimuraYoichi Ueta

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knockout
PCR
ELISA
PMA

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.