Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors

Scientific Reports
Koichiro OgawaHiroshi Arima

Abstract

Arginine-vasopressin (AVP) neurons exist in the hypothalamus, a major region of the diencephalon, and play an essential role in water balance. Here, we established the differentiation method for AVP-secreting neurons from human embryonic stem cells (hESCs) by recapitulating in vitro the in vivo embryonic developmental processes of AVP neurons. At first, the differentiation efficiency was improved. That was achieved through the optimization of the culture condition for obtaining dorsal hypothalamic progenitors. Secondly, the induced AVP neurons were identified by immunohistochemistry and these neurons secreted AVP after potassium chloride stimulation. Additionally, other hypothalamic neuropeptides were also detected, such as oxytocin, corticotropin-releasing hormone, thyrotropin-releasing hormone, pro-opiomelanocortin, agouti-related peptide, orexin, and melanin-concentrating hormone. This is the first report describing the generation of secretory AVP neurons derived from hESCs. This method will be applicable to research using disease models and, potentially, for regenerative medicine of the hypothalamus.

References

May 1, 1985·Clinical Endocrinology·T D WilliamsS L Lightman
Apr 1, 1997·Proceedings of the National Academy of Sciences of the United States of America·T FurukawaC L Cepko
Dec 31, 2003·Developmental Biology·Peter MayeLaura Grabel
Dec 4, 2004·Development·D Jonathan HorsfordRoderick R McInnes
Feb 8, 2005·Nature Neuroscience·Kiichi WatanabeYoshiki Sasai
May 29, 2007·Nature Biotechnology·Kiichi WatanabeYoshiki Sasai
Jun 2, 2007·Cell and Tissue Research·O von Bohlen Und Halbach
Jul 7, 2007·Physiological Reviews·Xiaoyan ZhuMichael G Rosenfeld
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Takafumi WatayaYoshiki Sasai
Feb 19, 2009·The Cerebellum·Catherine Vaillant, Denis Monard
Dec 3, 2010·Cold Spring Harbor Perspectives in Biology·Claudia S BarrosUlrich Müller
Jun 19, 2012·Cell Stem Cell·Tokushige NakanoYoshiki Sasai
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Taisuke KadoshimaYoshiki Sasai
Jan 3, 2015·The Journal of Clinical Investigation·Liheng WangRudolph L Leibel
Feb 12, 2015·Development·Florian T MerkleAlexander F Schier
Feb 20, 2015·Nature Communications·Atsushi KuwaharaYoshiki Sasai
Mar 31, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Joseph L BedontSeth Blackshaw
Jan 15, 2016·Nature Communications·Chikafumi OzoneYoshiki Sasai
Dec 23, 2016·Nagoya Journal of Medical Science·Hiroshi ArimaDaisuke Hagiwara

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Citations

Aug 4, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ali SeifinejadMehdi Tafti
Nov 19, 2020·Cellular and Molecular Life Sciences : CMLS·Ryusaku Matsumoto, Yutaka Takahashi
Mar 5, 2021·Development, Growth & Differentiation·Hajime OzakiHiroshi Arima
Jun 29, 2021·Frontiers in Physiology·Evanthia A Makrygianni, George P Chrousos
Jun 29, 2021·Developmental Biology·Brian GuyRobert J Johnston

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Methods Mentioned

BETA
PCR

Software Mentioned

FACS Diva

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