Vasorelaxant activity of Canavalia grandiflora seed lectin: A structural analysis

Archives of Biochemistry and Biophysics
Ito Liberato Barroso-NetoB S Cavada

Abstract

Lectins are comprised of a large family of proteins capable of the specific and reversible recognition of carbohydrates. Legume lectins, the most studied plant lectins, show high structural similarity, but with modifications that imply a variation in the intensity of some biological activities. In this work, the primary and tertiary structures of Canavalia grandiflora (ConGF) were determined. ConGF, a lectin isolated from C. grandiflora seeds, is able to induce relaxant activity in rat aortic rings. The complete sequence of ConGF comprises 237 amino acids. This particular protein has primary sequence variations commonly found in lectins from Dioclea and Canavalia genera. The protein structure was solved at 2.3 Å resolution by X-ray crystallography. An X-Man molecule was modeled into the carbohydrate recognition domain. Still, ConGF (30 and 100 μg mL(-1)) elicited 25% of vasorelaxation (IC50=34.48 ± 5.07 μg mL(-1)) in endothelialized aortic rings. A nonselective inhibitor of nitric oxide blocked ConGF relaxant effect, showing mediation by nitric oxide. Key distances between ConGF carbohydrate recognition domain residues were determined in order to explain this effect, in turn revealing some structural aspects that could differen...Continue Reading

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Citations

Jul 2, 2014·BioMed Research International·Mayron Alves VasconcelosMaria Olivia Pereira
Mar 8, 2016·Archives of Biochemistry and Biophysics·Alysson Chaves AlmeidaBenildo Sousa Cavada
Nov 8, 2015·Archives of Biochemistry and Biophysics·Ana Cecilia AlvesBenildo Sousa Cavada
Oct 30, 2016·International Journal of Biological Macromolecules·Vinicius Jose Silva OsterneBenildo Sousa Cavada
Jan 13, 2019·International Journal of Molecular Sciences·Annick BarrePierre Rougé
Apr 4, 2021·International Journal of Molecular Sciences·Anna Bartáková, Marie Nováková
Jan 29, 2017·International Journal of Biological Macromolecules·Vanir Reis Pinto-JuniorBenildo Sousa Cavada
Oct 27, 2015·International Journal of Biological Macromolecules·Ito L Barroso-NetoBenildo S Cavada
Aug 15, 2017·International Journal of Biological Macromolecules·Benildo Sousa CavadaRodrigo Bainy Leal
Apr 11, 2020·International Journal of Biological Macromolecules·Kyria S NascimentoBenildo S Cavada

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