Vasospastic action of hydrogen peroxide in human umbilical artery: relation to protein kinase C and calcium influx

Acta Obstetricia Et Gynecologica Scandinavica
Y OkataniY Sagara

Abstract

We have demonstrated that hydrogen peroxide (H2O2) potentiated vascular tension in human umbilical artery, perhaps by suppressing the synthesis of nitric oxide and prostacyclin. This study was conducted to evaluate whether the activation of protein kinase C (PKC) or voltage-dependent calcium channel mediated the vasospastic effect of H2O2. Helical sections of the umbilical artery were obtained from healthy pregnant women who delivered between 37th and 39th week of gestation. Changes in the maximal tension induced by prostaglandin F2 alpha (9 x 10(-7) M) were measured (isometric mechanical activity). Segments were treated with H2O2 (10(-6)-10(-4) M) alone or H2O2 after pretreatment with a scavenger of hydroxyl radicals (mannitol, 10(-2) M), an inhibitor of PKC (H-7, 6 x 10(-7) M). Effect of an activator of PKC (12-tetradecanoyl phorbol-13 acetate, TPA, 10(-6) M) on PG F2 alpha-induced tension was determined. Effects of H2O2 (10(-5) M) on the response of umbilical artery segments to an external calcium (10(-6)-10(-3) M) were determined. Vascular tension was potentiated by H2O2 in a concentration-dependent manner. Pretreatment with mannitol significantly suppressed the vasospastic effect of H2O2. Pretreatment with H-7 did not alte...Continue Reading

Citations

Sep 21, 2010·Antioxidants & Redox Signaling·Greg A Knock, Jeremy P T Ward
Jan 10, 2002·Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology·E KramplS Campbell
Sep 13, 2003·The Journal of Pharmacology and Experimental Therapeutics·Horacio VidrioAlejandro Baeza

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