Vba4p, a vacuolar membrane protein, is involved in the drug resistance and vacuolar morphology of Saccharomyces cerevisiae

Bioscience, Biotechnology, and Biochemistry
Miyuki Kawano-KawadaYoshimi Kakinuma

Abstract

In the vacuolar basic amino acid (VBA) transporter family of Saccharomyces cerevisiae, VBA4 encodes a vacuolar membrane protein with 14 putative transmembrane helices. Transport experiments with isolated vacuolar membrane vesicles and estimation of the amino acid contents in vacuoles showed that Vba4p is not likely involved in the transport of amino acids. We found that the vba4Δ cells, as well as vba1Δ and vba2Δ cells, showed increased susceptibility to several drugs, particularly to azoles. Although disruption of the VBA4 gene did not affect the salt tolerance of the cells, vacuolar fragmentation observed under high salt conditions was less prominent in vba4Δ cells than in wild type, vba1Δ, and vba2Δ cells. Vba4p differs from Vba1p and Vba2p as a vacuolar transporter but is important for the drug resistance and vacuolar morphology of S. cerevisiae.

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Citations

Mar 2, 2016·Bioscience, Biotechnology, and Biochemistry·Kunio ManabeYoshimi Kakinuma
Oct 3, 2018·Biological & Pharmaceutical Bulletin·Miyuki Kawano-KawadaTakayuki Sekito
Oct 18, 2019·Microbiology and Molecular Biology Reviews : MMBR·Frans BianchiBert Poolman
Mar 12, 2021·Bioscience, Biotechnology, and Biochemistry·Miyuki Kawano-KawadaTakayuki Sekito

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Methods Mentioned

BETA
ubiquitination
fluorescence microscopy
PCR
Fluorescence

Software Mentioned

MetaMorph

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