VCAM1 Labels a Subpopulation of Neural Stem Cells in the Adult Hippocampus and Contributes to Spatial Memory.

Stem Cell Reports
Dan-Ying WangXiao-Min Wang

Abstract

Active neural stem cells (aNSCs) and quiescent neural stem cells (qNSCs) are two distinct subpopulations found in the adult hippocampal dentate gyrus (DG). However, to date, no cell surface marker has been established to identify and profile qNSCs in the adult hippocampus. Here, we identified expression of vascular cell adhesion molecule 1 (VCAM1) on the cell surface of NSCs, through which we identified a previously unrecognized subpopulation of NSCs in the adult mouse DG. Interestingly, most VCAM1-expressing NSCs were largely quiescent. By injecting virus into Ai14 reporter mice to conduct lineage tracing in the adult DG, we confirmed that VCAM1-expressing cells were multipotent and capable of generating neurons and astrocytes. Furthermore, depletion of Vcam1 during the embryonic or adult stage impaired spatial learning and memory in mice, accompanied by a reduced number of radial glial-like cells and proliferating NSCs in the subgranular zone of Vcam1 knockout mice.

References

Dec 1, 1992·The Journal of Experimental Medicine·A S NeishT Collins
Dec 28, 1999·Protein Engineering·P Corish, C Tyler-Smith
Jul 19, 2005·Annual Review of Neuroscience·Guo-li Ming, Hongjun Song
Nov 4, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·M Soledad EspósitoAlejandro F Schinder
Mar 28, 2006·Neuroscience·I DriscollR J Sutherland
Apr 5, 2007·Nature Protocols·Charles V Vorhees, Michael T Williams
Apr 5, 2007·Nature Protocols·J Martin Wojtowicz, Nohjin Kee
Nov 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Diane C LagaceAmelia J Eisch
Dec 15, 2007·Nature Protocols·Nohjin KeePaul W Frankland
Feb 26, 2008·Cell·Chunmei ZhaoFred H Gage
Jun 3, 2008·Current Opinion in Neurobiology·Xin DuanHongjun Song
Jan 16, 2009·BMC Neuroscience·Lenneke W A van HooijdonkCarlos P Fitzsimons
Jun 27, 2009·Annual Review of Neuroscience·Arnold Kriegstein, Arturo Alvarez-Buylla
Feb 16, 2010·Neuron·Michael S Fanselow, Hong-Wei Dong
Feb 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tara L WalkerGerd Kempermann
Jan 6, 2016·Stem Cells·Elias GebaraNicolas Toni
Oct 19, 2016·Stem Cells·Katya ZelentsovaTal Burstyn-Cohen
Nov 5, 2016·Cell·J Tiago GonçalvesFred H Gage
May 5, 2017·Nature Reviews. Neuroscience·Christoph Anacker, René Hen
May 6, 2017·Frontiers in Biology·Fatih Semerci, Mirjana Maletic-Savatic
Aug 31, 2017·Cell Reports·Marlen KnoblochSebastian Jessberger
Feb 14, 2018·Science·Gregor-Alexander PilzSebastian Jessberger
May 12, 2018·Scientific Reports·Mythily GanapathiRandall H Morse
Jun 27, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Kylie A HuckleberryMichael R Drew

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