PMID: 11927012Apr 3, 2002Paper

VCP (p97) regulates NFkappaB signaling pathway, which is important for metastasis of osteosarcoma cell line

Japanese Journal of Cancer Research : Gann
Tatsuya AsaiKatsuyuki Aozasa

Abstract

In order to identify genes associated with metastasis, suppression subtractive hybridization (SSH) was performed using murine osteosarcoma cell line Dunn and its subline with higher metastatic potential, LM8. SSH revealed expression of the gene encoding valosin-containing protein (VCP; also known as p97) to be constitutively activated in LM8 cells, but it declined in Dunn cells when the cells became confluent. Because VCP is known to be involved in the ubiquitination process of Inhibitor-kappaBalpha (IkappaBalpha), an inhibitor of nuclear factor-kappaB (NFkappaB), whether VCP influences NFkappaB activation or not was examined by using VCP-transfected Dunn cells (Dunn/VCPs). When stimulated with tumor necrosis factor-alpha (TNFalpha), Dunn/VCPs showed constantly activated NFkappaB, although in the original Dunn cells and control vector transfectant (Dunn/Dunn-c) NFkappaB activation ceased when the cells became confluent. Western immunoblot analysis showed an increase of phosphorylated IkappaBalpha (p-IkappaBalpha) in the cytoplasm of confluent Dunn/Dunn-c cells compared to that of Dunn/VCPs. Therefore, decrease of p-IkappaBalpha degrading activity might be responsible for the decrease in NFkappaB activation. In vitro apoptosis a...Continue Reading

References

Oct 28, 1988·Science·P A Baeuerle, D Baltimore
Jan 10, 1980·Nature·G Poste, I J Fidler
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Mar 29, 1996·The Journal of Biological Chemistry·M RoffR T Hay
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Dec 1, 1995·Cancer Metastasis Reviews·A F ChambersA C Groom
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·S KoopA C Groom
Nov 1, 1996·Science·D J Van AntwerpI M Verma
Dec 4, 1996·Journal of the National Cancer Institute·I J Fidler, R Radinsky
Dec 15, 1996·Developmental Biology·F Fagotto, B M Gumbiner
Jan 31, 1998·The Journal of Clinical Investigation·M A SovakG E Sonenshein
May 14, 1998·International Journal of Cancer. Journal International Du Cancer·T AsaiH Yoshikawa
Aug 19, 1999·Journal of the National Cancer Institute·D R Welch, C W Rinker-Schaeffer
Sep 6, 2000·Urologic Oncology·A P ChristianoC W Rinker-Schaeffer
Nov 4, 2000·Journal of the National Cancer Institute·B A YoshidaC W Rinker-Schaeffer

❮ Previous
Next ❯

Citations

Mar 3, 2005·Journal of Molecular Biology·Byron DeLaBarre, Axel T Brunger
Jun 1, 2005·Experimental and Molecular Pathology·Li NanSamuel W French
Feb 25, 2009·The Journal of Biological Chemistry·Michael A GitchoNigel J Cairns
Feb 10, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Donatella CarpiRoberta Pastorelli
Dec 6, 2007·BMC Biochemistry·Robert Layfield, Barry Shaw
Jun 4, 2013·Cancer Letters·Delphine FessartEric Chevet
Jul 31, 2013·Nature Chemical Biology·Paola MagnaghiAntonella Isacchi
Jan 13, 2016·Journal of Molecular Histology·Sevil CayliTuncay Delibasi
Jul 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yan CuiDa Pang
Sep 5, 2006·Surgical Oncology·Nancy M Carroll, Sally E Carty
Jan 9, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xin Hua LongZhi Li Liu
Apr 17, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Nils KroegerPantuck A J
Apr 15, 2008·Biochimica Et Biophysica Acta·Esther OwsianowskiBirthe Fahrenkrog
Mar 21, 2007·Biochemical and Biophysical Research Communications·Binglin ZhangKatsuyuki Aozasa
Feb 1, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shinji YamamotoKatsuyuki Aozasa
Jun 28, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shinji YamamotoKatsuyuki Aozasa
Dec 15, 2004·Experimental and Molecular Pathology·Li NanSamuel W French
Apr 15, 2014·Molecular Cell·Veronique SchaefferIvan Dikic
Jan 13, 2006·Molecular & Cellular Proteomics : MCP·Chia-Hsin ChanLu-Ping Chow
May 12, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Keyvan BehnamElsa J Brochmann
Jan 22, 2009·Electrophoresis·Mariella Gruber-OlipitzGert Lubec
Feb 9, 2008·American Journal of Medical Genetics. Part a·Virginia E KimonisGiles D J Watts
Mar 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·James R CaveyRobert Layfield
Jan 13, 2012·Protein Science : a Publication of the Protein Society·Jeff Wereszczynski, J Andrew McCammon
Aug 29, 2006·Surgery Today·Shin-Ichi AsakaMasamitsu Onda
Oct 2, 2007·Expert Reviews in Molecular Medicine·Robert Layfield
Jul 20, 2005·The Journal of Biological Chemistry·Jon B KleinMadhavi J Rane
Jan 18, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Marie E BecknerIan F Pollack

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Related Papers

Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
Shinji YamamotoKatsuyuki Aozasa
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Shinji YamamotoKatsuyuki Aozasa
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Yuichi TsujimotoKatsuyuki Aozasa
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Shinji YamamotoKatsuyuki Aozasa
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Yuki KishidaAkira Myoui
© 2022 Meta ULC. All rights reserved