Vectorized Delivery of Alpha-GalactosylCeramide and Tumor Antigen on Filamentous Bacteriophage fd Induces Protective Immunity by Enhancing Tumor-Specific T Cell Response

Frontiers in Immunology
Rossella SartoriusPiergiuseppe De Berardinis

Abstract

We have exploited the properties of filamentous bacteriophage fd to deliver immunologically active lipids together with antigenic peptides. Filamentous bacteriophages resemble for size, capability to be permeable to blood vessels, and high density antigen expression, a nature-made nanoparticle. In addition, their major coat protein pVIII, which is arranged to form a tubular shield surrounding the phage genome, has a high content of hydrophobic residues promoting lipid association. We conjugated bacteriophages to alpha-GalactosylCeramide (α-GalCer), a lipid antigen-stimulating invariant natural killer T (iNKT) cells and capable of inducing their anti-tumoral activities. We found that bacteriophage fd/α-GalCer conjugates could repeatedly stimulate iNKT cells in vitro and in vivo, without inducing iNKT anergy. Moreover, co-delivery of α-GalCer and a MHC class I restricted tumor-associated antigenic determinant to antigen-presenting cells via bacteriophages strongly boosted adaptive CD8+ T cell response and efficiently delayed tumor progression. Co-delivery of a tumor antigen and iNKT-stimulatory lipid on the surface of filamentous bacteriophages is a novel approach to potentiate adaptive anti-cancer immune responses, overcoming th...Continue Reading

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Citations

May 1, 2020·Frontiers in Chemistry·Shuyao Lang, Xuefei Huang
Jan 29, 2020·Bioengineering & Translational Medicine·Krystina L Hess, Christopher M Jewell
Jun 8, 2019·Annual Review of Chemical and Biomolecular Engineering·Alexandra N Tsoras, Julie A Champion
Apr 9, 2020·Acta Biomaterialia·Prateek BhardwajRinti Banerjee

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Methods Mentioned

BETA
transfection
FACS
ELISA
flow cytometry
PMA
FCS

Software Mentioned

GraphPad Prism
DIVA
DIVA ( Data - Interpolating Variational Analysis )
GraphPad

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