VEGF-A gene promoter polymorphisms and microvascular complications in patients with essential hypertension

Clinical Biochemistry
Raffaele PalmirottaFiorella Guadagni

Abstract

We investigated the possible involvement of vascular endothelial growth factor (VEGF-A) gene promoter polymorphisms in essential hypertension (EH). 1225bp of the VEGF-A gene promoter were screened for polymorphisms using PCR amplification and direct DNA sequence analysis in 62 EH and 62 normotensive (HS) individuals. Circulating VEGF-A levels were determined by immunoassay. -152G/A (p=0.009) and -116G/A (p=0.016) polymorphisms were correlated to hypertension (p<0.05). Median platelet VEGF-A load in EH was 2.10fg/plt. Patients with microvascular complications (MC) had higher platelet VEGF-A load than those without (p=0.005). Multivariate analyses showed that -116 A allele was an independent predictor of microalbuminuria (p=0.014) and increased platelet VEGF-A load (p=0.009) in EH. Platelet VEGF-A load independently predicted MC (p=0.049) in addition to -116G/A polymorphism (p=0.035). Abnormal regulation of VEGF-A due to polymorphism at position -116 might represent a genetic factor for increased VEGF-A production and MC in EH.

References

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Citations

Dec 23, 2011·Journal of Human Hypertension·V C SandrimJ E Tanus-Santos
Aug 12, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ruhi KapahiNeeti Rajan Singh
Mar 10, 2011·Clinical Biochemistry·Marcelo Rizzatti Luizon, Valeria Cristina Sandrim
Sep 4, 2015·Thrombosis and Haemostasis·P FerroniM Roselli
Jul 15, 2015·Genetic Testing and Molecular Biomarkers·Young Ree Kim, Seung-Ho Hong
Jan 7, 2015·Pharmacogenomics·David Della-MorteMario Roselli
May 4, 2016·The Pharmacogenomics Journal·S P GampenriederR Greil

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