VEGF expression and cell apoptosis in NOD mouse retina.

International Journal of Ophthalmology
Cai-Rui Li, Shu-Guang Sun

Abstract

To investigate retinal vascular endothelial growth factor (VEGF) level and retinal cells apoptosis in the early stage of diabetic NOD mouse retina. Animals were divided into non-diabetes group, (control) (2-, 4-, 6-, 8- and 12-week sub-groups, n=30) and diabetes group (2-, 4-, 6-, 8- and 12-week sub-groups, n=30). Enzyme-linked immunosorbent assay (ELISA) was performed to detect VEGF level in both serum and retina. Transmission electron microscope method was used to examine retinal cell apoptosis. Compared with the control group, VEGF levels in serum and retina were increased significantly in the NOD group (12 weeks: 4.9±0.4µg/g vs 0.19±0.1µg/g in serum sample, P<0.01; 165±9µg/g vs 17±5µg/g in retinal sample, P<0.01). There exists a positive correlation between serum VEGF and retinal VEGF levels in the early diabetic NOD mice (γ=0.9902, P=0.001). The number of the cells apoptosis in the ganglion cells and endothelium can also been found increased significantly in the NOD group (P<0.01). The high VEGF expression may be contributed to increased retinal cells apoptosis. Many factors associated with retinal VEGF expression might involve in the early diabetes stage.

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Yan ke xue bao = Eye science
Cairui LiWengling Li
Research in Immunology
J F Bach, D Mathis
Nihon rinsho. Japanese journal of clinical medicine
H MoriyamaK Yokono
The American Journal of Pathology
G Papaccio
The American Journal of Pathology
E H LeiterD L Coleman
© 2021 Meta ULC. All rights reserved