VEGF gene therapy augments localized angiogenesis and promotes anastomotic wound healing: a pilot study in a clinically relevant animal model.

Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract
C Kristian EnestvedtBlair A Jobe

Abstract

Anastomotic leak related to ischemia is a source of significant morbidity and mortality in gastrointestinal surgery. The aim of this study was to apply growth factor gene transfection for the purpose of up-regulating angiogenesis, increasing anastomotic strength, and ultimately preventing dehiscence. An opossum esophagogastrostomy model was employed. The human vascular endothelial growth factor (VEGF(165)) gene was incorporated into a recombinant plasmid. The VEGF plasmid vector was then complexed with a cationic synthetic carrier, polyethyleneimine. Control animals received plasmid devoid of VEGF(165) (n = 6). The experimental group received VEGF(165) plasmid (n = 5). After esophagogastrectomy and gastric tubularization, plasmid was injected into the submucosa of the neoesophagus at the anastomotic site. Conduit arteriography was performed before and 10 days after injection. Euthanasia occurred on post-injection day 10 and the anastomosis was removed en bloc. A second group of animals treated with VEGF(165) were euthanized 30 and 37 days post injection. Blood flow was measured with laser-Doppler prior to euthanasia. Ex vivo anastomotic bursting pressure was performed. Tissue samples were procured for RNA extraction and von Wil...Continue Reading

References

Dec 16, 1992·Journal of the National Cancer Institute·N WeidnerG Gasparini
Nov 1, 1989·The Journal of Clinical Investigation·D T ConnollyJ Feder
Jun 1, 1995·American Journal of Surgery·J D Urschel
Feb 1, 1997·Experimental and Molecular Pathology·W F SeifertT Hendriks
Mar 20, 1999·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·R A ForseC D Mercer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 30, 2002·Annals of Surgical Oncology·Elisabeth A te VeldeInne H Borel Rinkes
Jul 27, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P B VermeulenL Y Dirix
Sep 26, 2002·World Journal of Surgery·Tatsuya MiyazakiKatsuhiko Tsukada
Nov 29, 2002·Gene Therapy·T Niidome, L Huang
May 3, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nikola ArsicMauro Giacca
Oct 8, 2003·Annals of Surgery·James D LuketichHiran C Fernando
May 29, 2004·Annals of Surgery·William C LineaweaverFeng Zhang
Aug 5, 2005·Gene Therapy·L K Medina-KauweS Hamm-Alvarez
Feb 7, 2006·Current Gene Therapy·John R OhlfestDavid A Largaespada
Jun 15, 2007·Human Gene Therapy·Serena ZacchignaGianmario Sambuceti
Nov 21, 2007·Surgical Endoscopy·Arman KilicJames D Luketich

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Citations

May 29, 2008·Cancer Metastasis Reviews·Dominique JenningsRobert J Gillies
Feb 27, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·L A E PosmaT Hendriks
Mar 17, 2010·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Edgard Engelman, Cécile Maeyens
Nov 26, 2009·Plastic and Reconstructive Surgery·Azra AshrafJeffrey Weinzweig
Apr 2, 2011·World Journal of Gastroenterology : WJG·Philipp Anton HolznerGoran Marjanovic
Nov 29, 2014·Plastic Surgery International·Gabriel DjedovicHildegunde Piza-Katzer
Oct 18, 2015·Surgical Innovation·Dimitrios NtourakisAnastasios Machairas
Apr 3, 2015·Vascular Medicine·Ozan L AbbasAyşe C Yazıcı
Oct 27, 2017·Acta chirurgica Belgica·Laura Christina LandesInes Gockel
Jan 26, 2010·World Journal of Surgery·Wolfgang SchröderChristian Gutschow
May 6, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Kevin M Reavis
Sep 9, 2011·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·C K EnestvedtB A Jobe
Apr 21, 2016·Langenbeck's Archives of Surgery·Gokhan AdasErdal Karaoz
Sep 15, 2012·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·M BludauW Schröder
Sep 28, 2010·The Annals of Thoracic Surgery·Marc BludauWolfgang Schröder
Dec 3, 2013·The Journal of Surgical Research·Emile RijckenPhilipp-Alexander Neumann

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