Abstract
To explore the effect of VEGF (vascular endothelial growth factor) on the vasculogenic mimicry (VM) formation of Choroidal Melanoma (CM) through PI3k signal pathway, to find novel targets for CM therapy. This research investigated the molecular mechanism of VEGF promoting VM formation of CM. First, we evaluated the expressions of VEGF in 20 CM specimens by immunohistochemical determination. Then we detected expressions of VEGF, AKT, MT1-MMP, MMP2, and MMP9 of OCM-1 in hypoxia. siRNA was used to inhibit the expression of VEGF, to realize the control of the VM formation. The VM formation was evaluated through wound healing assay, transwell assay, and apoptosis. And then we testify the correlation of the VM and the factors in protein and mRNA level preliminarily. VEGF protein was expressed in CM in all 20 cases of CM, especially along the VM. In hypoxia, the expression of VEGF in OCM-1 increased significantly. VEGF gene deletion reduced the proliferation, migration, and invasion of OCM-1. VEGF gene deletion impaired the expression of invasive associated genes like VEGF, p-AKT, AKT, MT1-MMP, MMP2, and MMP9. These results indicate that VEGF induce VM formation in CM by activating PI3K/AKT signaling pathway. VEGF promoted VM formatio...Continue Reading
References
Sep 18, 1997·Journal of the National Cancer Institute·A F Chambers, L M Matrisian
Apr 29, 1999·Annals of Medicine·V M Kähäri, U Saarialho-Kere
Sep 17, 1999·The American Journal of Pathology·A J ManiotisM J Hendrix
Oct 29, 1999·Journal of Cancer Research and Clinical Oncology·U GraevenD K Hossfeld
Aug 22, 2000·Nature·M BittnerV Sondak
Apr 6, 2001·The American Journal of Pathology·A K SoodM J Hendrix
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·M J HendrixR E Seftor
Mar 21, 2002·Nature Reviews. Cancer·Adrian L Harris
May 25, 2002·The British Journal of Surgery·Ian M LoftusM M Thompson
Jul 13, 2002·International Journal of Cancer. Journal International Du Cancer·Kazuo ShirakawaFumio Konishi
Jun 5, 2003·Nature Reviews. Cancer·Mary J C HendrixRichard E B Seftor
Jun 10, 2003·Breast Cancer Research : BCR·Kazuo ShirakawaHiro Wakasugi
Jun 12, 2003·World Journal of Gastroenterology : WJG·Manoj Kumar Gupta, Ren-Yi Qin
Mar 5, 2004·Cancer Research·Yusuke MizukamiDaniel C Chung
May 1, 2004·American Journal of Obstetrics and Gynecology·Anil K SoodMary J C Hendrix
Nov 26, 2004·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Robert Folberg, Andrew J Maniotis
Aug 18, 2005·Clinical Science·Hiroyuki Takahashi, Masabumi Shibuya
Dec 6, 2005·Angiogenesis·Melanie WeigandJohannes Waltenberger
Aug 8, 2008·Immunopharmacology and Immunotoxicology·Chandrasekaran Guruvayoorappan, Girija Kuttan
Dec 2, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Fan SuXiaochao Liu
Nov 26, 2009·Diseases of the Colon and Rectum·Coen I M BaetenCor G M I Baeten
Jan 20, 2015·Journal of Cellular and Molecular Medicine·Lili QiaoYuan Tian
Jan 26, 2016·Pharmacology & Therapeutics·Mary J C HendrixYi-Wen Chu
Jun 4, 2016·BMC Ophthalmology·Julia LükeAysegül Tura
Mar 23, 2017·Molecular Cancer·Daniel Delgado-BellidoF Javier Oliver
Citations
Oct 1, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xianjie JiangWei Xiong
May 20, 2020·Molecular Biology Reports·Nazila Fathi MaroufiMohammad Nouri
Nov 12, 2019·Frontiers in Oncology·Itzel Alejandra Hernández-RomeroErnesto Soto-Reyes
May 30, 2020·International Journal of Molecular Sciences·Mariusz L HartmanMalgorzata Czyz
Oct 31, 2020·International Journal of Molecular Sciences·Alexandru TirpeIoana Berindan-Neagoe
May 1, 2021·Cells·Ewelina DratkiewiczDorota Nowak
May 3, 2021·Archives of Biochemistry and Biophysics·Shao-Cheng LiuShyi-Gen Chen
May 8, 2021·Placenta·Peeyush K LalaChidambra Halari
May 10, 2021·Journal of Experimental & Clinical Cancer Research : CR·Simona D'AguannoAntonietta Moramarco
Aug 27, 2021·Translational Neuroscience·Yuqiang MaWeiping Li
Sep 4, 2021·Frontiers in Oncology·Bochao GengWenjuan Luo
Aug 31, 2021·Frontiers in Oncology·Yu ZhangJianping Shen