VEGF released from a fibrin biomatrix increases VEGFR-2 expression and improves early outcome after ischaemia-reperfusion injury

Journal of Tissue Engineering and Regenerative Medicine
Martina MoritzMartijn van Griensven

Abstract

Skeletal ischaemia-reperfusion (I-R) injury may influence patient outcome after severe vascular trauma or clamping of major vessels. The aim of this study was to observe whether locally applied vascular endothelial growth factor (VEGF) in fibrin could induce the expression of VEGF-receptor-2 (VEGFR-2) and improve the outcome after I-R injury. Transgenic mice expressing VEGFR-2 promoter-controlled luciferase were used for the assessment of VEGFR-2 expression. Ischaemia was induced for 2 h by a tension-controlled tourniquet to the hind limb, followed by 24 h of reperfusion. The animals were locally injected subcutaneously with fibrin sealant containing 20 or 200 ng VEGF; control animals received no treatment or fibrin sealant application. In vivo VEGFR-2 expression was quantified upon administration of luciferin at several observation times. For oedema and inflammation quantification, wet:dry ratio measurements and a myeloperoxidase assay of the muscle tissue were performed. Laser Doppler imaging showed that ischaemia was present and that the blood flow had returned to baseline levels after 24 h of reperfusion. VEGFR-2 expression levels in the fibrin + 200 ng VEGF were significantly higher than in all other groups. Granulocyte in...Continue Reading

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Citations

Oct 23, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Xuan ShiXin Lv
Feb 18, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Yanwen Li, Shikun Liu
May 23, 2021·In Vitro Cellular & Developmental Biology. Animal·Siti Nurnasihah Md HashimAhmad Azlina

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