VEGF stimulated the angiogenesis by promoting the mitochondrial functions

Oncotarget
Dongqing GuoXing Chen

Abstract

The vascular endothelial growth factor (VEGF) signaling pathway involved in angiogenesis which plays a pivotal role in normal development and also represents a major therapeutic target for tumors and intraocular neovascular disorders. The aims of the present study were to evaluate the effects of VEGF on endothelial cells and clarify the mechanism. Here, we showed that VEGF significantly stimulated the proliferation, migration and cell cycle of endothelial cells, and it also induced tube formation in vitro significantly. What's more, the mitochondrial functions were enhanced in response to VEGF, including mitochondrial oxidative respiration and intracellular ATP levels. The reactive oxygen species (ROS) production decreased, while the enzymes of ROS defence system, including catalase and glutathione peroxidase (GPX1), whose expression both increased in the VEGF stimulation. VEGF activated mammalian target of rapamycinm (mTOR) signaling pathway to promote the function of mitochondria. Rapamycin, the inhibitor of mTOR pathway could inhibit the proliferation and cell cycle induced by VEGF. In summary, our study identified that VEGF promoted the angiogenesis and provided evidence for mitochondria as new therapeutic target of VEGF si...Continue Reading

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Citations

Jan 6, 2018·Biomedicines·Dario DidonaCarmen Cantisani
Mar 23, 2018·Frontiers in Physiology·Lissette C Sánchez-ArangurenMarcos López
May 15, 2018·Frontiers in Pharmacology·Dongqing GuoXing Chen
Mar 20, 2020·Beilstein Journal of Nanotechnology·Gayathri KandasamyUma Maheswari Krishnan
Jun 4, 2020·Journal of Extracellular Vesicles·Roberta AngioniAntonella Viola
Nov 8, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Maki IwaiMamoru Tanaka
Jan 30, 2021·Cell and Tissue Research·MacKinsey Johnson, Chishimba Nathan Mowa
Apr 22, 2020·Cell Metabolism·Stéphanie HerkenneLuca Scorrano

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Methods Mentioned

BETA
flow cytometry
RNA-seq
Assay
reverse transcription PCR

Software Mentioned

Image Pro - Plus
Image J
SPSS

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