VEGFR2-targeted molecular imaging in the mouse embryo: an alternative to the tumor model

Ultrasound in Medicine & Biology
Janet M DenbeighF Stuart Foster

Abstract

As a tumor surrogate, the mouse embryo presents as an excellent alternative for examining the binding of angiogenesis-targeting microbubbles and assessing the quantitative nature of molecular ultrasound. We establish the validity of this model by developing a robust method to study microbubble kinetic behavior and investigate the reproducibility of targeted binding in the murine embryo. Vascular endothelial growth factor receptor 2 (VEGFR2)-targeted (MBV), rat immunoglobulin G2 (IgG2) control antibody-targeted (MBC) and untargeted (MBU) microbubbles were introduced into vasculature of living mouse embryos. Non-linear contrast-specific and B-mode ultrasound imaging, performed at 21 MHz with a Vevo-2100 scanner, was used to collect basic perfusion parameters and contrast mean power ratios for all bubble types. We observed a twofold increase (p < 0.001) in contrast mean power ratios for MBV (4.14 ± 1.78) compared with those for MBC (1.95 ± 0.78) and MBU (1.79 ± 0.45). Targeted imaging of endogenous endothelial cell surface markers in mouse embryos is possible with labeled microbubbles. The mouse embryo thus presents as a versatile model for testing the performance of ultrasound molecular targeting, where further development of qua...Continue Reading

References

Feb 22, 2001·Journal of the National Cancer Institute·M Höckel, P Vaupel
May 7, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Masayuki EndohYasufumi Kaneda
Oct 19, 2002·Circulation Research·Hideo Kusuoka, Julien I E Hoffman
May 25, 2004·American Journal of Physiology. Heart and Circulatory Physiology·E A V JonesM E Dickinson
Jun 3, 2004·Nature Reviews. Drug Discovery·Jonathan R Lindner
Dec 2, 2004·The British Journal of Radiology·H-D Liang, M J K Blomley
May 13, 2005·Physiology·Erica D Watson, James C Cross
Feb 25, 2006·The British Journal of Radiology·V R Stewart, P S Sidhu
Mar 21, 2006·ILAR Journal·Shathiyah KulandaveluS Lee Adamson
Nov 6, 2008·Congenital Anomalies·Makiko YamadaHiroki Otani
Mar 28, 2009·Methods : a Companion to Methods in Enzymology·Jens-Uwe Voigt
Jun 12, 2009·Medical & Biological Engineering & Computing·Alexander L Klibanov
Oct 22, 2009·Reproductive Sciences·Niamh CorriganFionnuala M Auliffe
Jan 5, 2010·Ultrasound in Medicine & Biology·Marios Lampaskis, Michalakis Averkiou
Jul 20, 2010·European Journal of Nuclear Medicine and Molecular Imaging·David Cosgrove, Nathalie Lassau
Feb 24, 2011·Investigative Radiology·Christopher R AndersonJoshua J Rychak
Apr 5, 2011·Cold Spring Harbor Protocols·Monica D GarciaMary E Dickinson
Feb 22, 2012·Translational Research : the Journal of Laboratory and Clinical Medicine·Yoichi Inaba, Jonathan R Lindner
Mar 1, 2012·Circulation Research·Benjamin B BartelleDaniel H Turnbull
Mar 7, 2012·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Fabian KiesslingTwan Lammers
Jul 28, 2012·International Journal of Molecular Sciences·Siver A MoestueRune Hansen

❮ Previous
Next ❯

Citations

Dec 19, 2015·Ultrasound in Medicine & Biology·Sarah E SheltonPaul A Dayton
Mar 3, 2015·IEEE Transactions on Bio-medical Engineering·Avinoam David Bar-ZionF Stuart Foster
Apr 7, 2015·International Journal of Pharmaceutics·Yusuke OdaKazuo Maruyama
Nov 12, 2019·Ultrasound in Medicine & Biology·Judy YanChristine E M Démoré

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Cancer Imaging

Imaging techniques, including CT and MR, have become essential to tumor detection, diagnosis, and monitoring. Here is the latest research on cancer imaging.