Abstract
Vascular smooth muscle cell (VMSC) proliferation is an essential component of myointimal hyperplasia, which is implicated in the failure of 30% to 50% of vascular interventions, such as coronary angioplasty and peripheral vein grafting. We have shown that cells derived from stenotic lesions in infrainguinal vein grafts were significantly more resistant than controls to growth inhibition by heparin. In a prospective study, we correlated antiproliferative responses to heparin in vitro with graft patency after 1 year. Sixty-two patients with infrainguinal vein grafts were entered into a graft surveillance program for > or = 1 year. At operation, saphenous vein segments were explanted for VSMC culture. Cell proliferation in response to fetal calf serum was later determined in the presence and absence of heparin. In 35 cell cultures, including 13 from the above-mentioned patients, [3H]heparin binding was also estimated. VSMCs from patients with patent grafts were significantly more sensitive to growth inhibition by heparin than cells from patients with stenoses (median, 54% versus 20.9%, P<0.001), and [3H]heparin binding was strongly correlated with inhibition of proliferation (r=0.81). Responsiveness to heparin in cultured VSMCs is...Continue Reading
References
Feb 17, 1977·Nature·A W Clowes, M J Karnowsky
Oct 1, 1976·Carbohydrate Research·E A Johnson, B Mulloy
Jan 11, 1992·Annals of Vascular Surgery·D L CullF N Parent
May 1, 1992·European Journal of Vascular Surgery·P R TaylorA N Nicolaides
May 1, 1991·Journal of the American College of Cardiology·B F WallerT Peters
Oct 1, 1990·The British Journal of Surgery·P R TaylorR E Houston
Jan 1, 1989·Journal of Cellular Physiology·T J ResinkF R Bühler
Jun 30, 1988·The New England Journal of Medicine·W McBrideL D Hillis
Jan 1, 1987·Differentiation; Research in Biological Diversity·O SkalliG Gabbiani
Apr 1, 1985·Experimental and Molecular Pathology·G R Campbell, J H Campbell
Jul 1, 1985·Journal of Cellular Physiology·J J CastellotM J Karnovsky
Nov 1, 1981·Atherosclerosis·J H Chamley-Campbell, G R Campbell
Aug 1, 1994·Journal of Cellular Physiology·T BârzuM Pascal
Sep 1, 1994·Journal of Vascular Surgery·E MunroJ Wolfe
Feb 6, 1993·Lancet·P ChanP Sever
Mar 1, 1993·European Journal of Vascular Surgery·P ChanJ Wolfe
Apr 29, 1993·Nature·R Ross
Mar 1, 1993·Haemostasis·Y P AuA W Clowes
Feb 1, 1997·Cardiology Clinics·G S MintzM B Leon
Citations
Dec 7, 2010·Acta Pharmacologica Sinica·Duk-hee ChunYoung Lan Kwak
Jun 29, 1999·British Journal of Pharmacology·M K PatelA D Hughes
Mar 27, 2010·PloS One·Santiago RedondoTeresa Tejerina
May 6, 2004·Journal of Hypertension·Rodney J DilleyJohn F Bertram
Oct 26, 2016·Journal of Vascular Surgery·Antonio V SterpettiMarco Ventura
Mar 25, 2009·Journal of Vascular Surgery·Richard D KenagyAlexander W Clowes
Jun 14, 2005·International Journal of Urology : Official Journal of the Japanese Urological Association·Kazumasa MatsumotoShiro Baba
Jun 9, 2021·Biofabrication·Faraz FazalNorbert Radacsi