Vein graft stenosis and the heparin responsiveness of human vascular smooth muscle cells

Circulation
J S RefsonP Sever

Abstract

Vascular smooth muscle cell (VMSC) proliferation is an essential component of myointimal hyperplasia, which is implicated in the failure of 30% to 50% of vascular interventions, such as coronary angioplasty and peripheral vein grafting. We have shown that cells derived from stenotic lesions in infrainguinal vein grafts were significantly more resistant than controls to growth inhibition by heparin. In a prospective study, we correlated antiproliferative responses to heparin in vitro with graft patency after 1 year. Sixty-two patients with infrainguinal vein grafts were entered into a graft surveillance program for > or = 1 year. At operation, saphenous vein segments were explanted for VSMC culture. Cell proliferation in response to fetal calf serum was later determined in the presence and absence of heparin. In 35 cell cultures, including 13 from the above-mentioned patients, [3H]heparin binding was also estimated. VSMCs from patients with patent grafts were significantly more sensitive to growth inhibition by heparin than cells from patients with stenoses (median, 54% versus 20.9%, P<0.001), and [3H]heparin binding was strongly correlated with inhibition of proliferation (r=0.81). Responsiveness to heparin in cultured VSMCs is...Continue Reading

References

Oct 1, 1976·Carbohydrate Research·E A Johnson, B Mulloy
May 1, 1992·European Journal of Vascular Surgery·P R TaylorA N Nicolaides
May 1, 1991·Journal of the American College of Cardiology·B F WallerT Peters
Oct 1, 1990·The British Journal of Surgery·P R TaylorR E Houston
Jun 30, 1988·The New England Journal of Medicine·W McBrideL D Hillis
Jan 1, 1987·Differentiation; Research in Biological Diversity·O SkalliG Gabbiani
Apr 1, 1985·Experimental and Molecular Pathology·G R Campbell, J H Campbell
Jul 1, 1985·Journal of Cellular Physiology·J J CastellotM J Karnovsky
Nov 1, 1981·Atherosclerosis·J H Chamley-Campbell, G R Campbell
Mar 1, 1993·European Journal of Vascular Surgery·P ChanJ Wolfe

❮ Previous
Next ❯

Citations

May 6, 2004·Journal of Hypertension·Rodney J DilleyJohn F Bertram
Oct 26, 2016·Journal of Vascular Surgery·Antonio V SterpettiMarco Ventura
Mar 25, 2009·Journal of Vascular Surgery·Richard D KenagyAlexander W Clowes
Jun 14, 2005·International Journal of Urology : Official Journal of the Japanese Urological Association·Kazumasa MatsumotoShiro Baba
Jun 9, 2021·Biofabrication·Faraz FazalNorbert Radacsi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiology Journals

Discover the latest cardiology research in this collection of the top cardiology journals.