Velocity valleys enable efficient capture and spatial sorting of nanoparticle-bound cancer cells

Nanoscale
Justin D BesantShana O Kelley

Abstract

The development of strategies for isolating rare cells from complex matrices like blood is important for a wide variety of applications including the analysis of bloodborne cancer cells, infectious pathogens, and prenatal testing. Due to their high colloidal stability and surface-to-volume ratio, antibody-coated magnetic nanoparticles are excellent labels for cellular surface markers. Unfortunately, capture of nanoparticle-bound cells at practical flow rates is challenging due to the small volume, and thus low magnetic susceptibility, of magnetic nanoparticles. We have developed a means to capture nanoparticle-labeled cells using microstructures which create pockets of locally low linear velocity, termed velocity valleys. Cells that enter a velocity valley slow down momentarily, allowing the magnetic force to overcome the reduced drag force and trap the cells. Here, we describe a model for this mechanism of cell capture and use this model to guide the rational design of a device that efficiently captures rare cells and sorts them according to surface expression in complex matrices with greater than 10,000-fold specificity. By analysing the magnetic and drag forces on a cell, we calculate a threshold linear velocity for capture ...Continue Reading

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Citations

Nov 17, 2015·Analytical Chemistry·Sanjin HosicAbigail N Koppes
Feb 11, 2016·Journal of the American Chemical Society·Mahmoud LabibShana O Kelley
Dec 9, 2015·Angewandte Chemie·Brenda J GreenShana O Kelley
May 6, 2015·ACS Applied Materials & Interfaces·Tina Saberi SafaeiShana O Kelley
Aug 25, 2015·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Ja Hye MyungSeungpyo Hong
May 20, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Nidal MuhannaShana O Kelley
May 27, 2015·Analytical Chemistry·Reza M MohamadiShana O Kelley
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Jul 12, 2017·ACS Applied Materials & Interfaces·Mahla PoudinehShana O Kelley
Jun 21, 2017·Chemical Society Reviews·Joshua M JacksonSteven A Soper
Apr 27, 2018·Chemistry : a European Journal·Mashooq KhanJin-Ming Lin
Apr 24, 2020·Cancer Medicine·Laan Luo, Yongqing He
May 26, 2018·The Analyst·Hyungseok ChoKi-Ho Han
Feb 1, 2018·Nature Biomedical Engineering·Mahla PoudinehShana O Kelley

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