Vendor effects on murine gut microbiota influence experimental abdominal sepsis

The Journal of Surgical Research
Tobias HilbertSven Klaschik

Abstract

Experimental animal models are indispensable components of preclinical sepsis research. Reproducible results highly rely on defined and invariant baseline conditions. Our hypothesis was that the murine gut microbiota varies among different distributors of laboratory animals and that these variations influence the phenotype of abdominal sepsis derived from a bacterial inoculum model (intraperitoneal stool injection). Male C57BL/6 mice (8-wk old) purchased from Charles River (CR), Janvier (J), and Harlan (H) were sacrificed, and the bacterial composition of feces was analyzed using CHROMagar orientation medium. Stool was injected intraperitoneally into CR mice, followed by clinical observation and gene expression analysis. Experiments were repeated 16 mo later under the same conditions. Stool analysis revealed profound intervendor differences in bacterial composition, mainly regarding Staphylococcus aureus and Bacillus licheniformis. Mice challenged with CR as well as H feces developed significantly higher severity of disease and died within the observation period, whereas stool from J mice did not induce any of these symptoms. Real-time polymerase chain reaction revealed corresponding results with significant upregulation of pro...Continue Reading

Citations

Sep 28, 2018·Journal of Leukocyte Biology·Mathew A HugginsSara E Hamilton
May 16, 2019·Viruses·Torben Sølbeck RasmussenDennis Sandris Nielsen
Jul 16, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katherine T FayCraig M Coopersmith
Jun 24, 2017·Frontiers in Cellular and Infection Microbiology·Totta EhretFrank Seeber
Mar 3, 2020·Frontiers in Immunology·Jan HorakMartin Matejovic
Jun 24, 2020·Nature Ecology & Evolution·Amar SarkarPhilip W J Burnet
Apr 10, 2020·Ci Ji Yi Xue Za Zhi = Tzu-chi Medical Journal·Chun-Yao ChenHan-Chen Ho
Oct 8, 2020·Current Protocols in Immunology·Frances V SjaastadThomas S Griffith
Feb 7, 2021·Research in Veterinary Science·Torben Sølbeck RasmussenAxel Kornerup Hansen
Jan 14, 2020·Pharmacology & Therapeutics·Folkert SteinhagenChristian Bode
Mar 11, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Aaron C Ericsson, Craig L Franklin

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