Venezuelan Equine Encephalitis Virus Induces Apoptosis through the Unfolded Protein Response Activation of EGR1

Journal of Virology
Alan BaerKylene Kehn-Hall

Abstract

Venezuelan equine encephalitis virus (VEEV) is a previously weaponized arthropod-borne virus responsible for causing acute and fatal encephalitis in animal and human hosts. The increased circulation and spread in the Americas of VEEV and other encephalitic arboviruses, such as eastern equine encephalitis virus and West Nile virus, underscore the need for research aimed at characterizing the pathogenesis of viral encephalomyelitis for the development of novel medical countermeasures. The host-pathogen dynamics of VEEV Trinidad donkey-infected human astrocytoma U87MG cells were determined by carrying out RNA sequencing (RNA-Seq) of poly(A) and mRNAs. To identify the critical alterations that take place in the host transcriptome following VEEV infection, samples were collected at 4, 8, and 16 h postinfection and RNA-Seq data were acquired using an Ion Torrent PGM platform. Differential expression of interferon response, stress response factors, and components of the unfolded protein response (UPR) was observed. The protein kinase RNA-like endoplasmic reticulum kinase (PERK) arm of the UPR was activated, as the expression of both activating transcription factor 4 (ATF4) and CHOP (DDIT3), critical regulators of the pathway, was alte...Continue Reading

References

Jan 7, 1998·Immunological Reviews·D GriffinP Desprès
Aug 27, 1999·Journal of Neurovirology·B A SchoneboomF B Grieder
Mar 14, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·B A SchoneboomF B Grieder
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·C V RamanaG R Stark
Oct 18, 2002·Journal of Cellular Physiology·Gerald Thiel, Giuseppe Cibelli
Jun 18, 2003·The Journal of General Virology·S Saha, P N Rangarajan
Aug 13, 2003·Bioinformatics·Keith A BaggerlyC Marcelo Aldaz
Dec 4, 2003·Annual Review of Entomology·Scott C WeaverJuan-Carlos Navarro
Aug 5, 2005·The Journal of Pharmacology and Experimental Therapeutics·Frank G BottoneThomas E Eling
Mar 31, 2006·Virology Journal·Michael P MuehlenbeinGeorge V Ludwig
Nov 14, 2006·Bioinformatics·S Falcon, R Gentleman
Sep 27, 2007·FEMS Immunology and Medical Microbiology·James KoterskiLuis DaSilva
Nov 21, 2007·Seminars in Cell & Developmental Biology·Jyoti D Malhotra, Randal J Kaufman
Nov 22, 2007·International Journal of Cancer. Journal International Du Cancer·Mandy WagnerIngo Tamm
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Dec 17, 2009·Acta Neuropathologica·Michael V Sofroniew, Harry V Vinters
Dec 24, 2009·Biosecurity and Bioterrorism : Biodefense Strategy, Practice, and Science·Erin ReichertJoseph Larsen
Mar 23, 2010·Biochemical and Biophysical Research Communications·Manish BhomiaRadha Maheshwari
Apr 24, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Yan FanJohnny J He
Jun 17, 2010·Veterinary Pathology·K E Steele, N A Twenhafel
Mar 10, 2011·The Journal of Biological Chemistry·José Peña, Eva Harris
Aug 3, 2011·Biotechnology and Bioengineering·Anirikh ChakrabartiJeffrey D Varner
Apr 13, 2012·PloS One·Kylene Kehn-HallFatah Kashanchi
May 25, 2012·Journal of Virology·Svetlana AtashevaIlya Frolov
Dec 4, 2012·International Journal of Molecular Sciences·Judith-Irina Pagel, Elisabeth Deindl
Jan 29, 2013·Cellular and Molecular Life Sciences : CMLS·Neysan DonnellyAfshin Samali

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Citations

Aug 29, 2018·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Victoria K Baxter, Mark T Heise
Jan 12, 2019·Frontiers in Microbiology·Lindsay LundbergKylene Kehn-Hall
Sep 17, 2019·Journal of Virological Methods·Viktoriya V KeyerAlexandr V Shustov
Apr 1, 2021·PLoS Neglected Tropical Diseases·Zhiyuan YaoShirit Einav
Jun 3, 2021·Viruses·Bibha DahalKylene Kehn-Hall
Sep 1, 2021·Glia·Savannah G SimsGordon P Meares

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Datasets Mentioned

BETA
L01442
PRJNA300864

Methods Mentioned

BETA
RNA-Seq
reverse transcription-PCR
fluorescence microscopy
protein folding
glycosylation

Software Mentioned

GSEA
GeneMania system
VEEV
CLC bio Genomics Workbench
Reactome
edgeR Bioconductor
Nikon NIS
GOStats
- Elements AR Analysis

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