Venomics, lethality and neutralization of Naja kaouthia (monocled cobra) venoms from three different geographical regions of Southeast Asia

Journal of Proteomics
Kae Yi TanNget Hong Tan

Abstract

Previous studies showed that venoms of the monocled cobra, Naja kaouthia from Thailand and Malaysia are substantially different in their median lethal doses. The intraspecific venom variations of N. kaouthia, however, have not been fully elucidated. Here we investigated the venom proteomes of N. kaouthia from Malaysia (NK-M), Thailand (NK-T) and Vietnam (NK-V) through reverse-phase HPLC, SDS-PAGE and tandem mass spectrometry. The venom proteins comprise 13 toxin families, with three-finger toxins being the most abundant (63-77%) and the most varied (11-18 isoforms) among the three populations. NK-T has the highest content of neurotoxins (50%, predominantly long neurotoxins), followed by NK-V (29%, predominantly weak neurotoxins and some short neurotoxins), while NK-M has the least (18%, some weak neurotoxins but less short and long neurotoxins). On the other hand, cytotoxins constitute the main bulk of toxins in NK-M and NK-V venoms (up to 45% each), but less in NK-T venom (27%). The three venoms show different lethal potencies that generally reflect the proteomic findings. Despite the proteomic variations, the use of Thai monovalent and Neuro polyvalent antivenoms for N. kaouthia envenomation in the three regions is appropriat...Continue Reading

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Citations

Aug 22, 2015·Journal of Biochemical and Molecular Toxicology·Diganta DasRobin Doley
Mar 15, 2016·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Kae Yi TanNget Hong Tan
May 11, 2016·Toxicon : Official Journal of the International Society on Toxinology·Cassandra M ModahlStephen P Mackessy
Jun 4, 2016·Toxicon : Official Journal of the International Society on Toxinology·Kitisak SintiprungratKavi Ratanabanangkoon
Jul 16, 2016·Journal of Proteomics·Esther Lai Har TangNget Hong Tan
Oct 25, 2016·Journal of Proteomics·Line P LauridsenJosé María Gutiérrez
Sep 13, 2016·Toxicon : Official Journal of the International Society on Toxinology·Choo Hock TanNget Hong Tan
Apr 9, 2016·PLoS Neglected Tropical Diseases·Kavi RatanabanangkoonNget Hong Tan
Mar 30, 2016·The American Journal of Tropical Medicine and Hygiene·Kin Ying WongNget Hong Tan
Sep 21, 2017·Toxins·Theo Tasoulis, Geoffrey K Isbister
Jan 19, 2019·PLoS Neglected Tropical Diseases·Felipe Silva-de-FrançaDenise V Tambourgi
Apr 20, 2017·Toxins·Anjana SilvaGeoffrey K Isbister
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Aug 3, 2018·Cellular and Molecular Life Sciences : CMLS·Anjana SilvaGeoffrey K Isbister
Feb 6, 2019·Toxins·Syafiq Asnawi Zainal AbidinRakesh Naidu
Sep 19, 2020·Transactions of the Royal Society of Tropical Medicine and Hygiene·Choo Hock TanKae Yi Tan

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