Venomics of Remipede Crustaceans Reveals Novel Peptide Diversity and Illuminates the Venom's Biological Role

Toxins
Björn Marcus von ReumontRonald A Jenner

Abstract

We report the first integrated proteomic and transcriptomic investigation of a crustacean venom. Remipede crustaceans are the venomous sister group of hexapods, and the venom glands of the remipede Xibalbanus tulumensis express a considerably more complex cocktail of proteins and peptides than previously thought. We identified 32 venom protein families, including 13 novel peptide families that we name xibalbins, four of which lack similarities to any known structural class. Our proteomic data confirm the presence in the venom of 19 of the 32 families. The most highly expressed venom components are serine peptidases, chitinase and six of the xibalbins. The xibalbins represent Inhibitory Cystine Knot peptides (ICK), a double ICK peptide, peptides with a putative Cystine-stabilized α-helix/β-sheet motif, a peptide similar to hairpin-like β-sheet forming antimicrobial peptides, two peptides related to different hormone families, and four peptides with unique structural motifs. Remipede venom components represent the full range of evolutionary recruitment frequencies, from families that have been recruited into many animal venoms (serine peptidases, ICKs), to those having a very narrow taxonomic range (double ICKs), to those unique ...Continue Reading

References

Aug 14, 1999·Biochimica Et Biophysica Acta·Y SokolovB L Kagan
Mar 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Martin FanenbruckJohann Wolfgang Wägele
Oct 14, 2005·Molecular Biology and Evolution·Daniel H Huson, David Bryant
Jul 31, 2007·Developmental and Comparative Immunology·Klara StensvågOlaf B Styrvold
Oct 27, 1961·Science·M E Pilson, P B Taylor
Jan 26, 2008·BMC Evolutionary Biology·Tobias H Kloepper, Daniel H Huson
Apr 12, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Adriana Muhlia-AlmazánFernando L García-Carreño
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jul 16, 2008·Toxicon : Official Journal of the International Society on Toxinology·Glenn F KingGraham M Nicholson
Mar 19, 2009·Journal of Molecular Evolution·Bryan G FryJ A Norman
May 22, 2009·Journal of Proteomics·Bryan G FryCamila Renjifo
Jun 10, 2009·Bioinformatics·Heng Li1000 Genome Project Data Processing Subgroup
Sep 15, 2009·PLoS Computational Biology·Steve HoffmannJörg Hackermüller
Oct 29, 2009·BMC Bioinformatics·Mira V Han, Christian M Zmasek
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Feb 27, 2010·BMC Evolutionary Biology·Nicolas MontagnéJean-Yves Toullec
Oct 6, 2010·Genome Biology·Camilla M WhittingtonWesley C Warren
Feb 1, 2011·Bioinformatics·Robert Schmieder, Robert Edwards
Feb 22, 2011·Bioinformatics·Diego DarribaDavid Posada
Feb 22, 2011·Bioinformatics·Stefan GötzAna Conesa
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
Jun 1, 2011·PloS One·Marco T NeiberStefan Koenemann
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Nov 1, 2011·PLoS Computational Biology·Sean R Eddy
Feb 1, 2012·The Journal of Biological Chemistry·Prakash G BalasubramanianSuat Ozbek
Mar 27, 2012·Briefings in Bioinformatics·Iain MilneDavid Marshall
Aug 14, 2012·Journal of Proteome Research·José R A dos Santos PintoMario Sergio Palma
Oct 2, 2012·Annual Review of Entomology·Glenn F King, Margaret C Hardy
Aug 22, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexandra SchwarzMichalis Kotsyfakis
Jan 24, 2014·Bioinformatics·Philip JonesSarah Hunter
Feb 18, 2014·Bioinformatics·Yinlong XieJun Wang
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
May 23, 2014·Molecular Biology and Evolution·Eivind A B UndheimBryan G Fry
Jun 4, 2014·Journal of Proteomics·Valérie CornetCéline Zatylny-Gaudin
Sep 7, 2014·Genome Biology and Evolution·Björn Marcus von ReumontChristoph Bleidorn
Nov 8, 2014·Science·Bernhard MisofXin Zhou
Dec 24, 2014·Toxins·Bjoern M von ReumontRonald A Jenner
Jan 3, 2015·Toxicon : Official Journal of the International Society on Toxinology·Ursula Castro de OliveiraInácio de Loiola Meirelles Junqueira-de-Azevedo
Jan 21, 2015·Journal of Proteomics·Kristian W SanggaardJan J Enghild
Feb 28, 2015·Toxins·Eivind A B UndheimGlenn F King
Apr 22, 2015·Scientific Reports·Zhirui LiuYonghua Ji
May 29, 2015·PloS One·Karen Luna-RamírezLourival D Possani
Jul 2, 2015·Toxins·Sébastien J M Moreau, Sassan Asgari
Aug 26, 2015·Toxicon : Official Journal of the International Society on Toxinology·Nathalia Batista DiasMario Sergio Palma
Oct 16, 2015·Toxicon : Official Journal of the International Society on Toxinology·Jason MacranderMarymegan Daly
Jan 26, 2016·Toxins·Axel TouchardAlain Dejean
Apr 30, 2016·PLoS Neglected Tropical Diseases·Paula Beatriz SantiagoJaime M Santana
May 12, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Eivind A B UndheimGlenn F King
Jul 28, 2016·Toxins·Inácio L M Junqueira-de-AzevedoStephen P Mackessy
Aug 16, 2016·Molecular Biology and Evolution·Helena Safavi-HemamiBaldomero M Olivera
Sep 18, 2016·Integrative and Comparative Biology·Stephen P Mackessy, Anthony J Saviola
Mar 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Irène R ChassagnonGlenn F King
Apr 4, 2017·Current Biology : CB·Nicholas R CasewellBryan G Fry
Jun 13, 2017·Current Biology : CB·Martin SchwentnerGonzalo Giribet

Citations

Aug 10, 2019·Molecular Biology and Evolution·Ronald A JennerEivind A B Undheim
Sep 8, 2017·Expert Review of Proteomics·Samuel D RobinsonGlenn F King
Jul 26, 2018·High-throughput·David Wilson, Norelle L Daly
Jul 28, 2019·Marine Drugs·Santos Ramírez-CarretoClaudia Rodríguez-Almazán
Sep 1, 2018·Science·Mandë HolfordRaymond S Norton
Mar 7, 2021·Toxins·Stephanie Mouchbahani-Constance, Reza Sharif-Naeini

Datasets Mentioned

BETA
SRR5483223
SRS744741
GCBC01000000

Methods Mentioned

BETA
Illumina
454 sequencing
Illumina sequencing
Dissection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.