Venous stasis-induced fibrinolysis prevents thrombosis in mice: role of α2-antiplasmin.

Blood
Satish SinghG L Reed

Abstract

Stasis of venous blood triggers deep vein thrombosis by activating coagulation, yet its effects on the fibrinolytic system are not fully understood. We examined the relationship between stasis, fibrinolysis, and the development of experimental venous thrombosis. Effects of stasis-induced deep vein thrombosis and fibrinolysis on thrombosis were examined by inferior vena cava ligation in congenic mice with and without α2-antiplasmin (α2AP), the primary inhibitor of plasmin. Venous thrombus weights were measured and thrombus composition was determined by Martius scarlet blue and immunofluorescence staining. Venous thrombi from α2AP+/+ mice contained plasminogen activators, plasminogen activator inhibitor-1, plasminogen, and α2AP, which changed with thrombus age. Normal, α2AP+/+ mice developed large, occlusive thrombi within 5 hours after ligation; thrombi were even larger in plasminogen-deficient mice (P < .001). No significant thrombus formation was seen in α2AP-/- mice (P < .0001) or in α2AP+/+ mice treated with an α2AP-inactivating antibody (P < .001). Venous stasis activated fibrinolysis, measured by D-dimer levels, in α2AP-/- mice vs α2AP+/+ mice (P < .05). Inhibition of fibrinolysis by the indirect plasmin inhibitor ε-aminoc...Continue Reading

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Citations

Apr 6, 2020·Journal of Thrombosis and Thrombolysis·Giuseppe Colucci, Dimitrios A Tsakiris
Jan 12, 2021·Frontiers in Cardiovascular Medicine·Satish SinghGuy L Reed
Feb 19, 2021·Seminars in Respiratory and Critical Care Medicine·Hannah StevensNoel Chan
Jun 8, 2021·HemaSphere·Robert L Medcalf, Charithani B Keragala
Jul 16, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Ghadir AlkarithiRobert A S Ariëns

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