Ventral specification and perturbed boundary formation in the mouse midbrain in the absence of Hedgehog signaling.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Jennifer L FogelSeema Agarwala

Abstract

Although Hedgehog (HH) signaling plays a critical role in patterning the ventral midbrain, its role in early midbrain specification is not known. We examined the midbrains of sonic hedgehog (Shh) and smoothened (Smo) mutant mice where HH signaling is respectively attenuated and eliminated. We show that some ventral (Evx1+) cell fates are specified in the Shh-/- mouse in a Ptc1- and Gli1-independent manner. HH-independent ventral midbrain induction was further confirmed by the presence of a Pax7-negative ventral midbrain territory in both Shh-/- and Smo-/- mice at and before embryonic day (E) 8.5. Midbrain signaling centers are severely disrupted in the Shh-/- mutant. Interestingly, dorsal markers are up-regulated (Wnt1, Gdf7, Pax7), down-regulated (Lfng), or otherwise altered (Zic1) in the Shh-/- midbrain. Together with the increased cell death seen specifically in Shh-/- dorsal midbrains (E8.5-E9), our results suggest specific regulation of dorsal patterning by SHH, rather than a simple deregulation due to its absence.

References

Jul 1, 1982·The Anatomical Record·A G Jacobson, P P Tam
Mar 30, 1995·Nature·G R Martin
Sep 1, 1995·Mechanisms of Development·L Bally-CuifM Wassef
Nov 14, 1996·Nature·V MarigoC J Tabin
Oct 23, 1997·Nature·I Rodriguez, K Basler
Aug 4, 1999·Trends in Genetics : TIG·C Dahmann, K Basler
Dec 4, 2001·Genes & Development·P W Ingham, A P McMahon
Nov 8, 2002·Development·Seema Agarwala, Clifton W Ragsdale
Aug 5, 2003·EMBO Reports·John Jacob, James Briscoe
Aug 15, 2003·Development·Iain PattenMarysia Placzek
Sep 23, 2003·Developmental Biology·Artem JarovClaire Fournier-Thibault
Aug 6, 2004·Neuron·Mark ZervasAlexandra L Joyner
Feb 15, 2005·Developmental Biology·Karin SchlichtingChristian Dahmann
Mar 5, 2005·Nature Neuroscience·Dimitris BourikasEsther T Stoeckli
Jul 5, 2006·Development·Grigoriy GutinJean M Hébert
Apr 17, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Galina V Aglyamova, Seema Agarwala
May 18, 2007·Development·Roy D BaylySeema Agarwala
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

Mar 16, 2013·Brain Structure & Function·J A Moreno-BravoE Puelles
Aug 15, 2014·Stem Cell Research·Yu-Rim KwonJong-Sun Kang
Sep 21, 2011·Progress in Neurobiology·Jennifer A Thompson, Mel Ziman
Apr 6, 2011·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chi-Kuang Leo WangCaroline N Dealy
Oct 2, 2012·Birth Defects Research. Part A, Clinical and Molecular Teratology·Robert M CabreraBogdan J Wlodarczyk
Jan 8, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Sandra Blaess, Siew-Lan Ang
Mar 21, 2009·Mechanisms of Development·Ariadna Perez-BalaguerSalvador Martinez
Jul 4, 2012·Developmental Biology·Roy D BaylySeema Agarwala
Mar 6, 2015·Frontiers in Neuroanatomy·Jesus E Martinez-LopezEduardo Puelles
Apr 18, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Verónica CompanyEduardo Puelles

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