Ventricular action potential adaptation to regular exercise: role of β-adrenergic and KATP channel function

Journal of Applied Physiology
Xinrui Wang, Robert H Fitts

Abstract

Regular exercise training is known to affect the action potential duration (APD) and improve heart function, but involvement of β-adrenergic receptor (β-AR) subtypes and/or the ATP-sensitive K+ (KATP) channel is unknown. To address this, female and male Sprague-Dawley rats were randomly assigned to voluntary wheel-running or control groups; they were anesthetized after 6-8 wk of training, and myocytes were isolated. Exercise training significantly increased APD of apex and base myocytes at 1 Hz and decreased APD at 10 Hz. Ca2+ transient durations reflected the changes in APD, while Ca2+ transient amplitudes were unaffected by wheel running. The nonselective β-AR agonist isoproterenol shortened the myocyte APD, an effect reduced by wheel running. The isoproterenol-induced shortening of APD was largely reversed by the selective β1-AR blocker atenolol, but not the β2-AR blocker ICI 118,551, providing evidence that wheel running reduced the sensitivity of the β1-AR. At 10 Hz, the KATP channel inhibitor glibenclamide prolonged the myocyte APD more in exercise-trained than control rats, implicating a role for this channel in the exercise-induced APD shortening at 10 Hz. A novel finding of this work was the dual importance of altered ...Continue Reading

References

Apr 1, 1975·The American Journal of Physiology·R H FittsJ O Holloszy
Apr 1, 1995·Journal of Applied Physiology·A J Woodiwiss, G R Norton
Oct 1, 1993·Cardiovascular Research·R B ClarkW R Giles
Nov 1, 1995·Medicine and Science in Sports and Exercise·R T ChattertonR C Hickson
Nov 15, 1996·Circulation·M H LehmannD J MacNeil
Oct 10, 1997·The American Journal of Physiology·E A MokelkeR L Moore
Aug 7, 1998·European Journal of Applied Physiology and Occupational Physiology·A J WoodiwissG R Norton
Mar 31, 1999·Free Radical Research·S AsamiH Kasai
Jul 17, 1999·The American Journal of Physiology·H M HimmelU Ravens
Feb 9, 2000·American Journal of Physiology. Endocrinology and Metabolism·Z Q SunI Klein
Apr 11, 2000·Journal of Molecular and Cellular Cardiology·P TaggartP Kallis
Sep 27, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A MoraskaM Fleshner
Mar 15, 2001·Journal of Applied Physiology·K N JewR L Moore
Jun 5, 2004·Medicine and Science in Sports and Exercise·Julie BarbierFrançois Carré
Aug 21, 2004·American Journal of Physiology. Heart and Circulatory Physiology·John P KonhilasLeslie A Leinwand
Jul 19, 2005·Progress in Biophysics and Molecular Biology·Chantal E Conrath, Tobias Opthof
Jun 10, 2006·Cardiovascular Research·Marcel A G van der HeydenTobias Opthof
Jan 16, 2007·Pharmacology, Biochemistry, and Behavior·Rebecca M CraftMegan K Pinckney
Feb 6, 2007·Medicine and Science in Sports and Exercise·Jeffrey F GorzekDawn A Lowe
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Ole J KemiUlrik Wisloff
May 2, 2008·International Journal of Biological Sciences·J Timothy Lightfoot
Oct 22, 2008·Methods : a Companion to Methods in Enzymology·R Madelaine ParedesJames D Lechleiter
Aug 18, 2009·American Journal of Physiology. Heart and Circulatory Physiology·George E Billman
May 11, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Spring R FarrellSusan E Howlett
Sep 22, 2010·Circulation·Stephan GielenVolker Adams
Mar 29, 2011·Journal of Molecular and Cellular Cardiology·Leonid V ZingmanDenice M Hodgson-Zingman
Jan 31, 2012·Acta Pharmacologica Sinica·Anthony Yiu Ho Woo, Rui-ping Xiao
May 4, 2013·Journal of Applied Physiology·Miguel Araujo Carneiro-JúniorJosé Geraldo Mill

❮ Previous
Next ❯

Citations

Aug 4, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Xinrui Wang, Robert H Fitts
Aug 8, 2018·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Alessandro GaspariniJuan-Jesús Carrero
May 28, 2020·Pflügers Archiv : European journal of physiology·Rajni SudhirAleksandar Jovanović
Oct 15, 2019·Frontiers in Physiology·Laura Martinez-MateuAdemuyiwa S Aromolaran
Nov 9, 2020·Mayo Clinic Proceedings·Biff F PalmerMatthew R Weir
Apr 11, 2021·Experimental Gerontology·Luke A Howlett, Matthew K Lancaster

❮ Previous
Next ❯

Software Mentioned

NIS
MetaMorph
MetaFluor
Clampex
Elements
GraphPad Prism

Related Concepts

Related Feeds

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.