Ventricular cell fate can be specified until the onset of myocardial differentiation

Mechanisms of Development
Simona Caporilli, Branko V Latinkić

Abstract

The mechanisms that govern specification of various cell types that constitute vertebrate heart are not fully understood. Whilst most studies of heart development have utilised the mouse embryo, we have used an alternative model, embryos of the frog Xenopus laevis, which permits direct experimental manipulation of a non-essential heart. We show that in this model pluripotent animal cap explants injected with cardiogenic factor GATA4 mRNA express pan-myocardial as well as ventricular and proepicardial markers. We found that cardiac cell fate diversification, as assessed by ventricular and proepicardial markers, critically depends on tissue integrity, as it is disrupted by dissociation but can be fully restored by inhibition of the BMP pathway and partially by Dkk-1. Ventricular and proepicardial cell fates can also be restored in reaggregated GATA4-expressing cells upon transplantation into a host embryo. The competence of the host embryo to induce ventricular and proepicardial markers gradually decreases with the age of the transplant and is lost by the onset of myocardial differentiation at the late tailbud stage (st. 28). The influence of the host on the transplant was not limited to diversification of cardiac cell fates, but...Continue Reading

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Citations

Feb 25, 2017·Development·Arjana PradhanDeborah Yelon
Nov 14, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Justyna Niderla-BieliŃskaAnna Ratajska

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Methods Mentioned

BETA
Fluorescence
PCR
transgenic

Software Mentioned

WMISH

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