PMID: 43544Jan 1, 1979

Verdohemoglobin as a substrate for proteases is applicable to assays over a wide pH range

Physiological Chemistry and Physics
T Murakami, T Murachi

Abstract

Verdohemoglobin, a heme-modified derivative readily obtained by ascorbic acid-coupled oxidation of oxyhemoglobin, was found to be a suitable substrate for protease with which assay can be carried out at all pH values. The advantage of verdohemoglobin over such popular substrates as alkali-and-urea-denatured hemoglobin and casein was demonstrated by a pH-profile study with Pronase E.

Related Concepts

Casein allergenic extract
Caseins
Peptide Hydrolases
Endopeptidases
Oxyhemoglobin Measurement
Proteolytic Enzyme
Oxidation
Ascorbic Acid Measurement
Substrate Specificity
Hemoglobin Measurement

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