Verification of complete bisulfite modification using Calponin-specific primer sets

Clinical Biochemistry
Ruethairat SriraksaTemduang Limpaiboon

Abstract

To verify complete bisulfite modification. Bisulfite modified DNA was screened by PCR with Calponin-specific primer sets prior to detecting methylation status of 14-3-3sigma by methylation-specific PCR. False positive methylation was obtained from incomplete bisulfite modification. The lower detection limit of wild type Calponin-specific primer is approximately 0.375 ng of unmodified DNA. Verification of complete bisulfite modification by Calponin-specific primer sets can reduce misinterpretation of methylation.

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Citations

Mar 15, 2014·PloS One·Brigitte L ThériaultTimothy W Corson
Oct 11, 2013·BioTechniques·Hernán G HernándezDiego A Forero
Mar 31, 2011·British Journal of Cancer·R SriraksaT Limpaiboon
Jan 24, 2018·Molecular Medicine Reports·Chaiyachet NanokTemduang Limpaiboon
Feb 6, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rattaya AmornpisuttTemduang Limpaiboon

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