Vernalization treatment induces site-specific DNA hypermethylation at the VERNALIZATION-A1 (VRN-A1) locus in hexaploid winter wheat

BMC Plant Biology
Abdul Rehman KhanClémentine Vitte

Abstract

Certain temperate species require prolonged exposure to low temperature to initiate transition from vegetative growth to flowering, a process known as vernalization. In wheat, winter cultivars require vernalization to initiate flowering, making vernalization requirement a trait of key importance in wheat agronomy. The genetic bases of vernalization response have been largely studied in wheat, leading to the characterization of a regulation pathway that involves the key gene VERNALIZATION1 (VRN1). While previous studies in wheat and barley have revealed the functional role of histone modification in setting VRN1 expression, other mechanisms might also be involved. Here, we were interested in determining whether the cold-induced expression of the wheat VRN-A1 gene is associated with a change in DNA methylation. We provide the first DNA methylation analysis of the VRN-A1 gene, and describe the existence of methylation at CG but also at non CG sites. While CG sites show a bell-shape profile typical of gene-body methylation, non CG methylation is restricted to the large (8.5 kb) intron 1, in a region harboring fragments of transposable elements (TEs). Interestingly, cold induces a site-specific hypermethylation at these non CG sites...Continue Reading

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Citations

Oct 25, 2014·BMC Genomics·Mélanie ThomasChristophe Tatout
Jul 17, 2018·Journal of Biochemistry·Hidetoshi Saze
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Jul 6, 2015·Plant Physiology·Taiko K ToTetsuji Kakutani
Sep 26, 2021·Nature Reviews. Genetics·Huiming ZhangJian-Kang Zhu

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Datasets Mentioned

BETA
AY747600
AY747598
AY747604.1
AY747604
AY747606

Methods Mentioned

BETA
acetylation
histone acetylation
PCR
restriction-PCR
electrophoresis
PCRs

Software Mentioned

ImageJ
SOFTGENETICS®
GEvo
Methyl Primer Express
BLASTN
Repeatmasker
Mutation Surveyor
Primer Express
TripleDirkC

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