Vernonia amygdalina-Induced Growth Arrest and Apoptosis of Breast Cancer (MCF-7) Cells

Pharmacology & Pharmacy
Clement YedjouPaul Tchounwou

Abstract

Breast cancer is the second leading cause of cancer-related deaths of women in the United States. Fortunately, the mortality rate from breast cancer has decreased in recent years due to an increased emphasis on early detection and more effective treatments. Although great advancements have been made in the treatment and control of cancer progression, significant deficiencies and room for improvement remain. The central objective of this research was to further determine the in vitro mechanisms of Vernonia amygdalina (VA) leaf extracts as an anticancer candidate for the treatment of breast cancer. To achieve our objective, MCF-7 cells were treated with different concentrations of VA for 24 hand 48 h. Cell viability, live and dead cells were determined by the means of trypan blue exclusion test. Live and dead cells were further evaluated by propidium iodine (PI) assay using the Cellometer Vision. Cell apoptosis was measured by flow cytometry assessment using annexin V/PI kit. Data obtained from the trypan blue test demonstrated that VA treatment reduces cell viability in a concentration- and time-dependent manner. Result of the PI assay showed a gradual increase in the population of necrotic cells (fluorescence positive cells) in...Continue Reading

References

Aug 30, 1994·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A H Wyllie
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jul 26, 2003·Journal of Natural Products·David J NewmanKenneth M Snader
Jan 20, 2009·International Journal of Environmental Research and Public Health·Clement YedjouPaul Tchounwou

❮ Previous
Next ❯

Citations

Jan 20, 2017·Nutrition and Cancer·Gilead Ebiegberi ForcadosPeace Mabeta
Sep 25, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·William JohnsonClement G Yedjou
Feb 3, 2018·BMC Complementary and Alternative Medicine·Wubayehu KahaliwEphrem Engidawork
May 3, 2018·Oncotarget·Adriana Harbuzariu, Ruben Rene Gonzalez-Perez
Jun 26, 2020·International Journal of Molecular Sciences·Ariane T MbemiPaul B Tchounwou
Jun 4, 2021·International Journal of Nanomedicine·Julia JosephNozlena Abdul Samad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis