Abstract
Airway mucus secretion is an essential innate immune response for host protection. However, overproduction and hypersecretion of mucus, mainly composed of MUC5AC, are significant risk factors in asthma and chronic obstructive pulmonary disease (COPD) patients. Previously, we reported that verproside, a catalpol derivative iridoid glycoside isolated from Pseudolysimachion rotundum var. subintegrum, is a potent anti-asthmatic candidate drug in vivo. However, the molecular mechanisms underlying the pharmacological actions of verproside remain unknown. Here, we found that verproside significantly reduces the expression levels of tumor necrosis factor alpha (TNF-α)-induced MUC5AC mRNA and protein by inhibiting both nuclear factor kappa B (NF-κB) transcriptional activity and the phosphorylation of its upstream effectors such as IκB kinase (IKK)β, IκBα, and TGF-β-activated kinase 1 (TAK1) in NCI-H292 cells. Moreover, verproside attenuated TNF-α-induced MUC5AC transcription more effectively when combined with an IKK (BAY11-7082) or a TAK1 (5z-7-oxozeaenol) inhibitor than when administered alone. Importantly, we demonstrated that verproside negatively modulates the formation of the TNF-α-receptor (TNFR) 1 signaling complex [TNF-RSC; TNF...Continue Reading
References
Feb 1, 1995·The American Journal of Medicine·N A HananiaS Kesten
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·K TakeyamaJ A Nadel
Oct 29, 2000·Cytokine·M G SmirnovaJ P Pearson
Apr 12, 2002·Histopathology·D A GronebergK F Chung
Oct 27, 2004·Histopathology·G CaramoriA Papi
Nov 26, 2004·Thorax·R A O'DonnellS J Wilson
Jun 7, 2005·American Journal of Respiratory and Critical Care Medicine·Hester van der VaartNick H T ten Hacken
Feb 4, 2006·American Journal of Respiratory Cell and Molecular Biology·Judith A VoynowMary C Rose
Apr 4, 2006·Annals of Medicine·Duncan F Rogers, Peter J Barnes
Apr 29, 2006·International Immunopharmacology·Sei-Ryang OhHyeong-Kyu Lee
Feb 16, 2008·Nature Reviews. Immunology·Peter J Barnes
Apr 4, 2008·Journal of Cellular Biochemistry·Jin Mi KimSeong Hwan Kim
Nov 5, 2008·Molecular and Cellular Biology·Ken BlackwellHasem Habelhah
Jan 7, 2009·Therapeutic Advances in Respiratory Disease·Amir SharafkhanehNicola Hanania
Feb 10, 2009·Chest·Judith A Voynow, Bruce K Rubin
May 16, 2009·Journal of Computational Chemistry·B R BrooksM Karplus
Aug 15, 2009·Cell Death and Differentiation·J Silke, R Brink
Dec 17, 2009·Molecular Cell·Tobias L HaasHenning Walczak
Mar 20, 2010·Cold Spring Harbor Perspectives in Biology·Alain Israël
Apr 24, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Tonio PeraHerman Meurs
Citations
Sep 21, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiuxia LuLin Tang
Apr 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ju-Hyun KimHye Suk Lee
Jan 25, 2018·Oxidative Medicine and Cellular Longevity·Yiran SunLin Tang
Jun 5, 2018·American Journal of Respiratory and Critical Care Medicine·Xiaoyun WangCaroline A Owen
Jun 21, 2019·Current Medicinal Chemistry·Liu-Qiang ZhangYi-Ming Li
Mar 24, 2018·Oncotarget·Yong ZouQiong Chen
Mar 7, 2020·Biomolecules & Therapeutics·Xin LiChoong Jae Lee
Jun 24, 2019·Allergy·Ilse M BoudewijnVictor Guryev
Sep 2, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen J Gurczynski, Bethany B Moore
Sep 28, 2018·Frontiers in Pharmacology·Su-Ui LeeSei-Ryang Oh
Oct 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingyuan Li, Zuguang Ye
Feb 28, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Lucas Amaral-MachadoEryvaldo Sócrates T Egito
Nov 22, 2016·PloS One·Jin ChoiHyun-Jun Lee
May 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kiram LeeHyun-Jun Lee
Nov 21, 2016·Inflammation·Jae-Won LeeKyoung-Seop Ahn
Jan 30, 2021·Molecules and Cells·Su Ui LeeTae-Don Kim
Mar 16, 2021·Animal Cells and Systems·Wan-Sik KimJae-Yong Lee
Aug 24, 2021·Journal of Asthma and Allergy·Xu ChenWenchun Xu