Versatile method for AFM-tip functionalization with biomolecules: fishing a ligand by means of an in situ click reaction

Nanoscale
Rakesh KumarYoko Yamakoshi

Abstract

A facile and universal method for the functionalization of an AFM tip has been developed for chemical force spectroscopy (CFS) studies of intermolecular interactions of biomolecules. A click reaction between tripod-acetylene and an azide-linker-ligand molecule was successfully carried out on the AFM tip surface and used for the CFS study of ligand-receptor interactions.

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Citations

Jun 28, 2018·Nanoscale·Yongliang YangLixin Dong
Jun 10, 2021·ACS Applied Materials & Interfaces·Alessandro FracassiYoko Yamakoshi
Feb 12, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·N TuccittoG Marletta

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