Versatile method to generate multiple types of micropatterns

Biointerphases
Felix Jakob SegererJoachim Oskar Rädler

Abstract

Micropatterning techniques have become an important tool for the study of cell behavior in controlled microenvironments. As a consequence, several approaches for the creation of micropatterns have been developed in recent years. However, the diversity of substrates, coatings, and complex patterns used in cell science is so great that no single existing technique is capable of fabricating designs suitable for all experimental conditions. Hence, there is a need for patterning protocols that are flexible with regard to the materials used and compatible with different patterning strategies to create more elaborate setups. In this work, the authors present a versatile approach to micropatterning. The protocol is based on plasma treatment, protein coating, and a poly(L-lysine)-grafted-poly(ethylene glycol) backfill step, and produces homogeneous patterns on a variety of substrates. Protein density within the patterns can be controlled, and density gradients of surface-bound protein can be formed. Moreover, by combining the method with microcontact printing, it is possible to generate patterns composed of three different components within one iteration of the protocol. The technique is simple to implement and should enable cell scienc...Continue Reading

References

Mar 1, 1969·Journal of Biomedical Materials Research·L Vroman, A L Adams
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R CarlssonE Ruoslahti
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kevin Kit ParkerDonald E Ingber
Sep 8, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Jason T SmithW Monty Reichert
Dec 17, 2004·Journal of the American Chemical Society·Jun NakanishiMizuo Maeda
Jan 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xingyu JiangGeorge M Whitesides
Sep 24, 2005·Nature Cell Biology·Manuel ThéryMichel Bornens
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Bryan A Langowski, Kathryn E Uhrich
Mar 22, 2006·Cell Motility and the Cytoskeleton·Manuel ThéryMichel Bornens
Dec 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Manuel ThéryMichel Bornens
Sep 26, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Lingyun LiuShaoyi Jiang
Jul 5, 2008·Biophysical Journal·Ilka B BischofsUlrich S Schwarz
May 22, 2009·Lab on a Chip·Ammar AziouneMatthieu Piel
Jun 10, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Isabelle BeaulieuJanine Mauzeroll
Dec 22, 2009·Lab on a Chip·Jonathan M BélisleSantiago Costantino
Feb 12, 2010·Cold Spring Harbor Protocols·Manuel Théry, Matthieu Piel
Dec 8, 2010·Biomaterials·Michael Junkin, Pak Kin Wong
Feb 8, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ravi A DesaiChristopher S Chen
Aug 2, 2011·Journal of Biotechnology·Jung-Ho KimJunhong Min
Dec 30, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Colin D EichingerVladimir Hlady
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qingzong TsengManuel Théry
Oct 4, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Laura Lara Rodriguez, Ian C Schneider
Jan 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Natalia M RodriguezChristopher S Chen
Mar 14, 2014·Soft Matter·Peter J F RöttgermannJoachim O Rädler
Sep 5, 2014·Biophysical Journal·Anna-Kristina MarelJoachim O Rädler
Feb 11, 2015·Biomaterials·Edmondo BattistaPaolo A Netti
Mar 24, 2015·Nature Cell Biology·Yee Han TeeAlexander D Bershadsky
Jul 22, 2015·Physical Review Letters·Felix J SegererJoachim O Rädler
Jul 24, 2015·Lab on a Chip·Mehrije FeriziJoachim O Rädler
Jan 23, 2007·Soft Matter·Sami Alom Ruiz, Christopher S Chen

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Citations

Jun 1, 2016·Scientific Reports·Christoph SchreiberJoachim O Rädler
Jan 25, 2018·Scientific Reports·Cécile M BidanAurélie Dupont
Jan 10, 2018·Cellular Microbiology·Julianne Mendi MuthinjaFriedrich Frischknecht
Dec 19, 2019·Integrative Biology : Quantitative Biosciences From Nano to Macro·A ReiserJ O Rädler
Dec 20, 2018·NPJ Systems Biology and Applications·Fabian FröhlichJan Hasenauer
Dec 23, 2019·Biophysical Journal·Alexandra FinkJoachim O Rädler
May 8, 2021·Colloids and Surfaces. B, Biointerfaces·L PasquardiniS Dirè
Oct 3, 2018·ACS Applied Materials & Interfaces·AshokKumar MeiyazhaganPulickel M Ajayan

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