Versatile polyion complex micelles for peptide and siRNA vectorization to engineer tolerogenic dendritic cells

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Naila MebarekPascale Louis-Plence

Abstract

Dendritic cells (DCs) are professional antigen-presenting cells that play a critical role in maintaining the balance between immunity and tolerance and, as such are a promising immunotherapy tool to induce immunity or to restore tolerance. The main challenge to harness the tolerogenic properties of DCs is to preserve their immature phenotype. We recently developed polyion complex micelles, formulated with double hydrophilic block copolymers of poly(methacrylic acid) and poly(ethylene oxide) blocks and able to entrap therapeutic molecules, which did not induce DC maturation. In the current study, the intrinsic destabilizing membrane properties of the polymers were used to optimize endosomal escape property of the micelles in order to propose various strategies to restore tolerance. On the first hand, we showed that high molecular weight (Mw) copolymer-based micelles were efficient to favor the release of the micelle-entrapped peptide into the endosomes, and thus to improve peptide presentation by immature (i) DCs. On the second hand, we put in evidence that low Mw copolymer-based micelles were able to favor the cytosolic release of micelle-entrapped small interfering RNAs, dampening the DCs immunogenicity. Therefore, we demonstr...Continue Reading

References

Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SinghD O'Hagan
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Jun 29, 2001·Trends in Immunology·H JonuleitA H Enk
Oct 13, 2004·Immunologic Research·Mu LiWei-Ping Min
Nov 16, 2004·Vaccine·Derek T O'HaganMichael Houghton
Mar 25, 2006·Journal of Immunological Methods·Alexander T PrechtelAlexander Steinkasserer
Aug 22, 2006·Immunobiology·Leonie M van DuivenvoordeRené E M Toes
Jul 14, 2007·Nature Reviews. Immunology·Adrian E Morelli, Angus W Thomson
Sep 25, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ankur SinghKrishnendu Roy
Oct 15, 2008·Biomaterials·Ariane BoudierSylvie Bégu
Feb 25, 2009·The Journal of Experimental Medicine·Caspar OhnmachtDavid Voehringer
Mar 11, 2009·Drug Development and Industrial Pharmacy·Ariane BoudierChristian Jorgensen
Apr 24, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jong Oh KimTatiana K Bronich
Oct 31, 2009·Scandinavian Journal of Immunology·C D PedersenA E Pedersen
Jan 21, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Rebecca Klippstein, David Pozo
Mar 5, 2010·International Reviews of Immunology·Catharien M U HilkensAngus W Thomson
Jun 17, 2010·Annals of the Rheumatic Diseases·Rachel A HarryCatharien M U Hilkens
Jun 1, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ariane BoudierSylvie Bégu
Oct 6, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Genc BashaPieter R Cullis
Feb 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fabienne DanhierVéronique Préat
Mar 20, 2012·Current Gene Therapy·Jean-Marc Humbert, Franck Halary
Apr 11, 2012·European Journal of Immunology·Dàlia Raϊch-ReguéFrancesc E Borràs
Feb 2, 2013·Transplantation Research·Aurélie MoreauMaria-Cristina Cuturi
Feb 2, 2013·Transplantation Research·Giada Amodio, Silvia Gregori
Feb 2, 2013·Current Opinion in Rheumatology·Catharina C Gross, Heinz Wiendl

❮ Previous
Next ❯

Citations

Aug 12, 2015·Colloids and Surfaces. B, Biointerfaces·Rajesh ThipparaboinaNalini R Shastri
Jan 20, 2021·ACS Biomaterials Science & Engineering·Farshid Eslami-KalijiM Rezaa Mohammadi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.