Versatility of tandem mass spectrometry for focused analysis of oxylipids

Journal of Mass Spectrometry : JMS
Tania PettaLúcia H Faccioli

Abstract

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the multiple reaction monitoring (MRM) scan mode has been the primary MS method applied for the target identification of specific and minor oxylipids in complex matrices, such as eicosanoids and docosanoids, which are potent lipid mediators derived from polyunsaturated fatty acid oxygenation. However, the high specificity of MRM can limit the detection of species with m/z MRM transitions not covered by the method. In addition to MRM, tandem-quadrupole mass analyzers enable other experiments to be conducted, by fragmenting ions via collision-induced dissociation process (CID). This paper presents the potential of tandem mass spectrometry for the focused analysis of oxylipids. We have successfully developed an LC-MS/MS method for the identification of precursor ions of m/z 115, a diagnostic product ion of 5-hydroxy- and 5-epoxy-fatty acids. As a proof of concept, the developed method was used to discover several oxylipids oxidized at C5 derived from arachidonic acid (C20 : 4) oxygenation in a hypothalamus rat extract that were not identified using the target MRM methodology. The proposed focused MS/MS-based approach in a tandem mass analyzer has proven to be a powerful ...Continue Reading

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Citations

Jun 9, 2016·Journal of Mass Spectrometry : JMS·Anelize BauermeisterLuiz Alberto Beraldo Moraes
Sep 3, 2017·Journal of Mass Spectrometry : JMS·Herbert J DiasAntônio E M Crotti
Aug 22, 2018·Scientific Data·Carlos Artério SorgiLúcia Helena Faccioli
Apr 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ivan LiakhAdriana Mika
Jan 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ivan LiakhAdriana Mika

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