Jan 6, 2005

Versican expression during skeletal/joint morphogenesis and patterning of muscle and nerve in the embryonic mouse limb

The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology
Holly E SnowAnthony A Capehart


Versican, an extracellular matrix proteoglycan, has been implicated in limb development and is expressed in precartilage mesenchymal condensations. However, studies have lacked precise spatial and temporal investigation of versican localization during skeletogenesis and its relationship to patterning of muscle and nerve during mammalian limb development. The transgenic mouse line hdf (heart defect), which bears a lacZ reporter construct disrupting Cspg2 encoding versican, allowed ready detection of hdf transgene expression through histochemical analysis. Hdf transgene expression in whole mount heterozygous embryos and localization of versican relative to cartilage, muscle, and nerve tissues in paraffin-embedded limb sections of wild-type embryos from 10.5-14 days postcoitum were evaluated by lacZ histochemistry, immunohistochemistry, and in situ hybridization. Versican was localized within precartilage condensations and nascent cartilages with expression diminishing during maturation of the cartilage model at later time points. Interestingly, versican remained highly expressed in developing synovial joint interzones, suggesting potential function for versican in joint morphogenesis. Isolated myoblasts, incipient skeletal muscle...Continue Reading

  • References9
  • Citations24


  • References9
  • Citations24


Mentioned in this Paper

Establishment and Maintenance of Localization
Mlc2f protein, zebrafish
Chondroitin Sulfate Proteoglycans
Articular System
Muscle Innervation, Function
Extracellular Matrix

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