Vertical distribution of bacterial and archaeal communities along discrete layers of a deep-sea cold sediment sample at the East Pacific Rise (approximately 13 degrees N).

Extremophiles : Life Under Extreme Conditions
Youxun LiYunshan Qin

Abstract

The community structure and vertical distribution of prokaryotes in a deep-sea (ca. 3,191 m) cold sediment sample (ca. 43 cm long) collected at the East Pacific Rise (EPR) approximately 13 degrees N were studied with 16SrDNA-based molecular analyses. Total community DNA was extracted from each of four discrete layers EPRDS-1, -2, -3 and -4 (from top to bottom) and 16S rDNA were amplified by PCR. Cluster analysis of DGGE profiles revealed that the bacterial communities shifted sharply between EPRDS-1 and EPRDS-2 in similarity coefficient at merely 49%. Twenty-three sequences retrieved from DGGE bands fell into 11 groups based on BLAST and bootstrap analysis. The dominant groups in the bacterial communities were Chloroflexi, Gamma proteobacteria, Actinobacterium and unidentified bacteria, with their corresponding percentages varying along discrete layers. Pairwise Fst (F-statistics) values between the archaeal clone libraries indicated that the archaeal communities changed distinctly between EPRDS-2 and EPRDS-3. Sequences from the archaeal libraries were divided to eight groups. Crenarchaea Marine Group I (MGI) was prevalent in EPRDS-1 at 83%, while Uncultured Crenarchaea group II B (UCII B) abounded in EPRDS-4 at 61%. Our result...Continue Reading

References

Jun 1, 1987·Microbiological Reviews·C R Woese
Feb 1, 1966·Journal of Theoretical Biology·E C Pielou
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M BarnsN R Pace
Jan 1, 1996·Microscopy Research and Technique·J M Larkin, M C Henk
Dec 24, 1997·Applied and Environmental Microbiology·M A MunsonT M Embley
Feb 6, 1998·FEMS Microbiology Reviews·F von WintzingerodeE Stackebrandt
Jun 11, 1998·Microbial Ecology·D P ChandlerJ K Fredrickson
Aug 1, 1997·Extremophiles : Life Under Extreme Conditions·C KatoK Horikoshi
Nov 5, 1999·Annual Review of Microbiology·J T Staley, J J Gosink
Nov 11, 1999·International Journal of Systematic Bacteriology·G BarbierG Raguénès
Apr 3, 2001·Applied and Environmental Microbiology·V M MadridA Y Chistoserdov
Aug 30, 2001·Applied and Environmental Microbiology·B J MacGregorD A Stahl
Aug 30, 2001·Applied and Environmental Microbiology·V T MarteinssonJ K Kristjánsson
Mar 28, 2002·Applied and Environmental Microbiology·Julie A HuberJohn A Baross
Jul 31, 2002·Applied and Environmental Microbiology·Andrew P Martin
Aug 1, 2002·International Journal of Systematic and Evolutionary Microbiology·M L MiroshnichenkoE A Bonch-Osmolovskaya
Aug 1, 2002·International Journal of Systematic and Evolutionary Microbiology·Karine AlainJean-Louis Birrien
Jun 6, 2003·Applied and Environmental Microbiology·Matthew O SchrenkJohn A Baross
Sep 18, 2003·International Journal of Systematic and Evolutionary Microbiology·Carine AudiffrinBernard Ollivier
Apr 7, 2004·Science·J Craig VenterHamilton O Smith
Apr 7, 2004·Applied and Environmental Microbiology·Ken TakaiKoki Horikoshi
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Nov 24, 2004·Journal of Basic Microbiology·Pattanop KanokratanaLily Eurwilaichitr
Mar 12, 2005·Extremophiles : Life Under Extreme Conditions·Peng WangFengping Wang
Oct 20, 2005·Journal of Bioscience and Bioengineering·Takahiro Kanagawa
Jan 18, 2006·Nature Reviews. Microbiology·Jennifer B Hughes MartinyJames T Staley
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Fumio InagakiBo B Jørgensen
Apr 7, 2006·The Journal of General and Applied Microbiology·Shizuka ArakawaChiaki Kato
Jul 22, 2006·Systematic and Applied Microbiology·Julia I K SchwarzRalf Conrad
Sep 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Fumio InagakiAntje Boetius

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Citations

Oct 5, 2010·Microbial Ecology·Peng WangChuanlun Zhang
May 28, 2010·Extremophiles : Life Under Extreme Conditions·Suman PradhanS Shivaji
Mar 7, 2014·World Journal of Microbiology & Biotechnology·Yang-Guo ZhaoFarhana Maqbool
Sep 8, 2011·Environmental Microbiology·Alan M Durbin, Andreas Teske

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