Vertical uniformity of cells and nuclei in epithelial monolayers

Scientific Reports
Srujana NeelamTanmay P Lele

Abstract

Morphological variability in cytoskeletal organization, organelle position and cell boundaries is a common feature of cultured cells. Remarkable uniformity and reproducibility in structure can be accomplished by providing cells with defined geometric cues. Cells in tissues can also self-organize in the absence of directing extracellular cues; however the mechanical principles for such self-organization are not understood. We report that unlike horizontal shapes, the vertical shapes of the cell and nucleus in the z-dimension are uniform in cells in cultured monolayers compared to isolated cells. Apical surfaces of cells and their nuclei in monolayers were flat and heights were uniform. In contrast, isolated cells, or cells with disrupted cell-cell adhesions had nuclei with curved apical surfaces and variable heights. Isolated cells cultured within micron-sized square wells displayed flat cell and nuclear shapes similar to cells in monolayers. Local disruption of nuclear-cytoskeletal linkages resulted in spatial variation in vertical uniformity. These results suggest that competition between cell-cell pulling forces that expand and shorten the vertical cell cross-section, thereby widening and flattening the nucleus, and the resis...Continue Reading

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Citations

Feb 28, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·C AllierJ-M Dinten
May 26, 2017·Journal of Cellular Physiology·Vincent J ToccoTanmay P Lele
Mar 30, 2018·International Journal of Molecular Sciences·Lauren SfakisJames Castracane
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Sep 19, 2020·International Journal of Molecular Sciences·Srujana NeelamYe Zhang
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Oct 6, 2021·ELife·Harold Fernando GómezDagmar Iber

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Methods Mentioned

BETA
confocal microscopy
transfection

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