Very bright orange fluorescent plants: endoplasmic reticulum targeting of orange fluorescent proteins as visual reporters in transgenic plants.

BMC Biotechnology
David G J MannC Neal Stewart

Abstract

The expression of fluorescent protein (FP) genes as real-time visual markers, both transiently and stably, has revolutionized plant biotechnology. A palette of colors of FPs is now available for use, but the diversity has generally been underutilized in plant biotechnology. Because of the green and far-red autofluorescent properties of many plant tissues and the FPs themselves, red and orange FPs (RFPs, and OFPs, respectfully) appear to be the colors with maximum utility in plant biotechnology. Within the color palette OFPs have emerged as the brightest FP markers in the visible spectra. This study compares several native, near-native and modified OFPs for their "brightness" and fluorescence, therefore, their usability as marker genes in transgenic plant tissues. The OFPs DsRed2, tdTomato, mOrange and pporRFP were all expressed under the control of the CaMV 35S promoter in agroinfiltration-mediated transient assays in Nicotiana benthamiana. Each of these, as well as endoplasmic reticulum (ER)-targeted versions, were stably expressed in transgenic Nicotiana tabacum and Arabidopsis thaliana. Congruent results were observed between transient and stable assays. Our results demonstrated that there are several adequate OFP genes avai...Continue Reading

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Citations

Aug 13, 2013·Plant Physiology·Balasubramaniam MuthukumarC Neal Stewart
May 5, 2012·BMC Biology·Alexander M JonesWolf B Frommer
Sep 28, 2013·BMC Research Notes·J Hollis RiceC Neal Stewart
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Methods Mentioned

BETA
transgenic
confocal microscopy
transgenics
Fluorescence
protein folding
PCR

Software Mentioned

tdTomato
SAS
Gene Design
mStrawberry
mOrange
GeMS

Related Concepts

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