Very mild disease phenotype of congenic CftrTgH(neoim)Hgu cystic fibrosis mice.

BMC Genetics
Balázs TóthB Tümmler

Abstract

A major boost to cystic fibrosis disease research was given by the generation of various mouse models using gene targeting in embryonal stem cells. Moreover, the introduction of the same mutation on different inbred strains generating congenic strains facilitated the search for modifier genes. From the original CftrTgH(neoim)Hgu mouse model with a divergent genetic background (129/Sv, C57BL/6, HsdOla:MF1) two inbred mutant mouse strains CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu had been generated using strict brother x sister mating. CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu mice were fertile and showed normal growth and lifespan. In this work the CftrTgH(neoim)Hgu insertional mutation was backcrossed from CF/3-CftrTgH(neoim)Hgu onto the inbred backgrounds C57BL/6J and DBA/2J generating congenic animals in order to clarify the differential impact of the Cftr mutation and the genetic background on the disease phenotype of the cystic fibrosis mutant mice. Clinical and electrophysiological features of the two congenic strains were compared with those of CF/1-CftrTgH(neoim)Hgu and CF/3-CftrTgH(neoim)Hgu and wild type controls. Under the standardized housing conditions of the animal facility, the four mouse strains C...Continue Reading

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Citations

Jun 17, 2011·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Martina WilkeBob J Scholte
Apr 22, 2010·The Journal of Clinical Investigation·Kuai YuH Criss Hartzell
Nov 9, 2011·Respiratory Research·Antje MunderBurkhard Tümmler
Oct 19, 2010·International Journal of Medical Microbiology : IJMM·Heike GrassméErich Gulbins
Apr 4, 2018·Respiratory Research·Alexandra McCarronDavid Parsons
Sep 23, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shashi ChillappagariMarkus O Henke

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Methods Mentioned

BETA
transgenic
PCR
genotyping

Software Mentioned

GraphPad Prism

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