Very rapid induction of a cold shock protein by temperature downshift in Thermus thermophilus

Biochemical and Biophysical Research Communications
Ryosuke MegaRyoji Masui

Abstract

A rapid temperature downshift induces the expression of many proteins termed 'cold-induced' proteins. Although some of these proteins are known to participate in metabolism, transcription, translation and protein folding, processes that are affected by cold stress, it has not yet been identified which proteins sense the temperature downshift. Here we analyzed the mRNA expression profiles of genes induced immediately following a temperature downshift in Thermus thermophilus HB8. The cold shock protein gene ttcsp2 displayed the most rapid and drastic increase in mRNA. ttcsp2 mRNA was induced at 30s after temperature downshift, although ttCSP2 protein was first detected at 10 min. A temperature-dependent secondary structure was predicted to form in the 5'-untranslated region, including the Shine-Dalgarno sequence, of ttcsp2 mRNA. Stabilization of this secondary structure at 45 degrees C was assumed to prevent degradation of ttcsp2 mRNA and to slow translation. Thus, ttCSP2 is considered to act as a 'thermosensor' during temperature downshift through changes in its secondary structure.

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Citations

Oct 27, 2012·FEBS Letters·Mercedes de la FuenteJosé Luis Martínez-Guitarte
Aug 9, 2016·Frontiers in Microbiology·Riikka Keto-TimonenHannu Korkeala
Jan 18, 2017·Archives of Microbiology·Preeti Ranawat, Seema Rawat
Jun 3, 2021·Microorganisms·Francis MuchaambaTaurai Tasara

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